Fine mapping of a murine growth locus to a 1.4-cM region and resolution of linked QTL
Fine mapping of a murine growth locus to a 1.4-cM region and resolution of linked QTL
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DOI：
10.1007/s00335-004-3046-0
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发表时间：
2004-06-01
期刊：
影响因子：
2.5
通讯作者：
Keightley, PD
中科院分区：
文献类型：
--
作者：
Christians, JK;Keightley, PD
以前的工作在C57BL/6J和DBA/2J小鼠之间的杂交中发现了一个影响小鼠大小(特别是尾长)的QTL，并将其定位在D1Mit30和D1Mit57之间的8 cM区域。本研究利用重组子代测试对该QTL进行了精细定位。从D1MR30和D1Mit57之间携带染色体重组的部分同源菌株的个体与DBA/2J交配，产生942个后代。在这个群体中发现了两个影响10周尾长的QTL：一个在D1Mit30(先前工作中估计的位置)远端9.7 cM处，以及D1Mit30附近影响较小的母亲。第二个群体(n=787)是从同一染色体片段杂合的后代测试群体中交配的兄弟姐妹产生的，只包括D1Mit265和D1Mit57之间的重组子。在后一个群体中，还检测到两个QTL：一个位于D1Mit30远端10.2 cM处，另一个位于作图区域远端(D1Mit150)。当两个群体一起分析时，中心QTL的估计位置在D1Mit30的远端10.2 cM，而远端QTL的证据略显着。中心QTL解释了大约7%的表型变异，其位置的95%可信区间(通过自举确定)是1.4 cM的区域，大致在D1Mit451到D1Mit219之间。中心QTL对3周龄和6周龄的尾长和体质量也有影响，但影响程度小于10周龄。
Previous work identified a QTL affecting murine size (particularly tail length) in a cross between C57BL/6J and DBA/2J mice and refined its location to an 8-cM region between D1Mit30 and D1Mit57. The present study used recombinant progeny testing to fine map this QTL. Individuals from a partially congenic strain carrying chromosomes recombinant between D1MR30 and D1Mit57 were mated to DBA/2J, generating 942 progeny. Two QTL affecting 10-week tail length were identified in this population: one at 9.7 cM distal to D1Mit30 (the position estimated in previous work), and mother of smaller effect near D1Mit30. A second population (n = 787) was generated by mating siblings from the progeny test population that were heterozygous for the same segment of chromosome including only recombinants between D1Mit265 and D1Mit57. In the latter population, two QTL were also identified: one at 10.2 cM distal to D1Mit30, and another of smaller effect at the distal end of the mapped region (at D1Mit150). When the two populations were analyzed together, the estimated location of the central QTL was 10.2 cM distal to D1Mit30 and there was marginally significant evidence of the distal QTL. The central QTL explained approximately 7% of the phenotypic variance, and the 95% confidence interval for its position (determined by bootstrapping) was a 1.4-cM region, approximately the region from D1Mit451 to D1Mit219. The central QTL also affected tail length and body mass at 3 and 6 weeks of age, but to a lesser degree than 10-week tail length.