Dental pulp stem cells as a multifaceted tool for bioengineering and the regeneration of craniomaxillofacial tissues.
Dental pulp stem cells as a multifaceted tool for bioengineering and the regeneration of craniomaxillofacial tissues.
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DOI：
10.3389/fphys.2015.00289
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发表时间：
2015
影响因子：
4
通讯作者：
Ibarretxe G
中科院分区：
文献类型：
--
作者：
Aurrekoetxea M;Garcia-Gallastegui P;Irastorza I;Luzuriaga J;Uribe-Etxebarria V;Unda F;Ibarretxe G
牙髓干细胞（DPSC）是一种神经嵴源性细胞，具有向沿着多种细胞谱系分化的优异能力，可用于细胞治疗。特别是，可以使用简单的体外方案实现DPSC的高效成骨/牙本质分化，使这些细胞成为未来治疗牙齿和牙周疾病的非常有吸引力和有前途的工具。在颅颌面器官中，牙齿和唾液腺是两个这样的情况下，其中使用DPSC的组织工程完全再生似乎是可能的，因为在过去十年的研究中，通过细胞重组技术，在两个器官的部分或全部再生的实验模型中取得了实质性进展。此外，DPSC似乎是神经组织再生的特别好的选择，包括损伤或横断的颅神经。在这种情况下，口腔似乎是使用DPSC的新再生疗法的绝佳试验场。然而，在这些细胞用于临床治疗之前，还需要解决许多问题和挑战。在这篇综述中，我们指出了DPSC的生物学方面的一些重要方面，其用于重建不同的颅颌面组织和器官，特别强调颅骨，神经，牙齿和唾液腺。我们提出了新的思路和策略，以充分利用DPSC的上述组织的生物工程的能力。
Dental pulp stem cells, or DPSC, are neural crest-derived cells with an outstanding capacity to differentiate along multiple cell lineages of interest for cell therapy. In particular, highly efficient osteo/dentinogenic differentiation of DPSC can be achieved using simple in vitro protocols, making these cells a very attractive and promising tool for the future treatment of dental and periodontal diseases. Among craniomaxillofacial organs, the tooth and salivary gland are two such cases in which complete regeneration by tissue engineering using DPSC appears to be possible, as research over the last decade has made substantial progress in experimental models of partial or total regeneration of both organs, by cell recombination technology. Moreover, DPSC seem to be a particularly good choice for the regeneration of nerve tissues, including injured or transected cranial nerves. In this context, the oral cavity appears to be an excellent testing ground for new regenerative therapies using DPSC. However, many issues and challenges need yet to be addressed before these cells can be employed in clinical therapy. In this review, we point out some important aspects on the biology of DPSC with regard to their use for the reconstruction of different craniomaxillofacial tissues and organs, with special emphasis on cranial bones, nerves, teeth, and salivary glands. We suggest new ideas and strategies to fully exploit the capacities of DPSC for bioengineering of the aforementioned tissues.