Assessment of the Effects of 6 Standard Rodent Diets on Binge-Like and Voluntary Ethanol Consumption in Male C57BL/6J Mice
Assessment of the Effects of 6 Standard Rodent Diets on Binge-Like and Voluntary Ethanol Consumption in Male C57BL/6J Mice
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DOI：
10.1111/acer.12773
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发表时间：
2015-08-01
影响因子：
3.2
通讯作者：
Thiele, Todd E.
中科院分区：
文献类型：
--
作者：
Marshall, Simon Alex;Rinker, Jennifer A.;Thiele, Todd E.
背景近年来，生物医学研究，特别是临床前动物研究中缺乏可重复性的问题引起了人们的广泛关注。这也是困扰酒精研究领域的一个问题，特别是在酒精使用障碍的动物模型中持续消费。一个经常被忽视的因素，可能会影响重现性是在临床前研究中使用的维护饮食。MethodsHere，2个完善的模型，酒精消费，在黑暗中饮用（DID）的程序和连续2瓶选择（C2BC）的范例，被用来确定饮食对乙醇（EtOH）消费的影响。雄性C57 BL/6 J小鼠给予从Purina LabDiet（R），Inc.（Prolab（（R））RMH 3000）或哈兰（（R））实验室公司。（Teklad Diets T.2916、T.2918、T.2920X、T.7912或T.8940）。一个单独的动物组被用来测试饮食对EtOH药代动力学的影响和腹膜内（IP）注射不同剂量的EtOH后的行为测量。结果在DID期间，食用哈兰饮食T. 2916（H2916）和T. 2920 X（H2920）的小鼠消耗显著较少的EtOH，并表现出较低的血液EtOH浓度（BEC）;然而，在C2BC期间，维持在哈兰T.7912（H7912）的动物消耗更多的EtOH，并且具有比其他饮食组更高的EtOH偏好。EtOH消耗水平并非源于酒精药代动力学的变化，因为IP给予EtOH的单独一组动物显示BEC无差异。然而，动物哈兰饮食T.2920 X（H2920）更敏感的酒精诱导的自发活动在一个开放的领域的任务。没有饮食依赖的差异被认为是在酒精诱导的镇静与翻正reflex.ConclusionsAlthough这些数据没有确定一个特定的机制，在一起，他们清楚地表明，维持饮食影响乙醇消费。研究界有责任考虑在方法中描述营养信息的重要性，这可能有助于减少实验室间的重现性问题。
BackgroundIn recent years, much attention has been given to the lack of reproducibility in biomedical research, particularly in preclinical animal studies. This is a problem that also plagues the alcohol research field, particularly in consistent consumption in animal models of alcohol use disorders. One often overlooked factor that could affect reproducibility is the maintenance diet used in preclinical studies.MethodsHerein, 2 well-established models of alcohol consumption, the drinking in the dark (DID) procedure and the continuous 2-bottle choice (C2BC) paradigm, were employed to determine the effects of diet on ethanol (EtOH) consumption. Male C57BL/6J mice were given 1 of 6 standard rodent chow diets obtained from Purina LabDiet((R)), Inc. (Prolab((R)) RMH 3000) or Harlan((R)) Laboratories, Inc. (Teklad Diets T.2916, T.2918, T.2920X, T.7912, or T.8940). A separate group of animals were used to test dietary effects on EtOH pharmacokinetics and behavioral measures following intraperitoneal (IP) injections of various doses of EtOH.ResultsMice eating Harlan diets T.2916 (H2916) and T.2920X (H2920) consumed significantly less EtOH and exhibited lower blood EtOH concentrations (BECs) during DID; however, during C2BC, animals maintained on Harlan T.7912 (H7912) consumed more EtOH and had a higher EtOH preference than the other diet groups. EtOH consumption levels did not stem from changes in alcohol pharmacokinetics, as a separate group of animals administered EtOH IP showed no difference in BECs. However, animals on Harlan diet T.2920X (H2920) were more sensitive to alcohol-induced locomotor activity in an open-field task. No diet-dependent differences were seen in alcohol-induced sedation as measured with loss of righting reflex.ConclusionsAlthough these data do not identify a specific mechanism, together, they clearly show that the maintenance diet impacts EtOH consumption. It is incumbent upon the research community to consider the importance of describing nutritional information in methods, which may help decrease interlaboratory reproducibility issues.