The lack of relationship between an endothelin-1 gene polymorphism (Ala288ser) and incidence of hypertension: a retrospective cohort study among Japanese workers.
The lack of relationship between an endothelin-1 gene polymorphism (Ala288ser) and incidence of hypertension: a retrospective cohort study among Japanese workers.
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DOI：
10.2188/jea.14.129
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发表时间：
2004-07
影响因子：
4.7
通讯作者：
Kurozawa Y
中科院分区：
文献类型：
--
作者：
Kaetsu A;Kishimoto T;Osaki Y;Okamoto M;Fukumoto S;Kurozawa Y
背景：一些病例对照关联研究揭示了一些内皮素-1 （ET-1）基因多态性与血压之间的关系。由于没有关于ET-1基因多态性与高血压发病率之间关系的报道，我们通过回顾性队列研究检验了新的ET-1基因多态性（外显子5中的G862T / Ala288Ser）与高血压发病率之间的关系。方法：研究对象为日本岛根县某公司的日籍员工。采用聚合酶链反应对抗双对引物法，分析其与工人血液中提取的基因组DNA的多态性。根据每隔6年进行两次定期健康检查的结果，在排除了1992年患有高血压的人群后，研究人群于1998年根据血压和抗高血压治疗分为两组。结果：在922例（男性540例，女性382例）研究人群中，高血压发病率为133例（男性93例，女性40例）。在单因素分析中，Ala/Ser和Ser/Ser对Ala/Ala的比值比分别为0.98(95%可信区间[CI]): 0.7-1.4)和0.79 （95% CI: 0.4-1.6）。在调整了性别、年龄、体重指数、血清总胆固醇、空腹血糖、吸烟和饮酒习惯的多变量分析中，Ala/Ser和Ser/Ser对Ala/Ala的比值比分别为0.97 （95% CI: 0.7-1.4）和0.75 （95% CI: 0.4-1.5）。结论：本研究中ET-1基因多态性似乎与日本工人的高血压发病率无关。
BACKGROUND: Some case-control association studies revealed the relationship between some endothelin-1 (ET-1) gene polymorphisms and blood pressure. Because no report was available about the relationship between any ET-1 gene polymorphism and incidence of hypertension, we examined the relationship between novel ET-1 gene polymorphism (G862T / Ala288Ser in exon 5) and incidence of hypertension by a retrospective cohort study. METHODS: The subjects were Japanese workers at a company in Shimane Prefecture in Japan. The polymorphism with genome DNA extracted from the blood of the workers was analyzed using the polymerase chain reaction confronting two pair primers method. According to the results of two regular health checkups with a 6-year interval, the study population was divided into two groups by blood pressure and antihypertensive treatment in 1998, after excluding people who had hypertension in 1992. RESULTS: There were 133 (93 males and 40 females) incidences of hypertension observed among the study population of 922 (540 males and 382 females). In the univariate analysis, odds ratios o Ala/Ser and Ser/Ser against Ala/Ala were 0.98 (95% confidence interval [CI]): 0.7-1.4) and 0.79 (95% CI: 0.4-1.6), respectively. In the multivariate analysis adjusted for sex, age, body mass index, serum total cholesterol, fasting blood sugar, and smoking and drinking habits, odds ratios for Ala/Ser and Ser/Ser against Ala/Ala were 0.97 (95% CI: 0.7-1.4) and 0.75 (95% CI: 0.4-1.5), respectively. CONCLUSIONS: The ET-1 gene polymorphism in this study did not seem to be associated with the incidence of hypertension among the Japanese workers.