VIRAL-E1 AND VIRAL-E2 PROTEINS SUPPORT REPLICATION OF HOMOLOGOUS AND HETEROLOGOUS PAPILLOMAVIRAL ORIGINS
VIRAL-E1 AND VIRAL-E2 PROTEINS SUPPORT REPLICATION OF HOMOLOGOUS AND HETEROLOGOUS PAPILLOMAVIRAL ORIGINS
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DOI：
10.1073/pnas.89.13.5799
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发表时间：
1992-07-01
影响因子：
11.1
通讯作者：
CHOW, LT
中科院分区：
文献类型：
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作者：
CHIANG, CM;USTAV, M;CHOW, LT
我们已经表明，E1和E2蛋白的人乳头瘤病毒11型（HPV-11）是必不可少的，以支持复制的同源病毒起源（ori）在瞬时复制试验，类似于牛乳头瘤病毒1型（BPV-1）的报告。出乎意料的是，来自HPV-11或BPV-1的E1和E2蛋白的匹配或甚至混合组合在上皮或成纤维细胞谱系的人、猴和啮齿动物细胞系中复制了ori，尽管效率不同。任何一组病毒蛋白也能够启动许多其他人和动物乳头瘤病毒的含ori质粒的复制。因此，乳头瘤病毒的顺式元件和反式因子之间的相互作用比乳多空病毒家族的其他成员，猿猴病毒40和多瘤病毒，其中大的肿瘤抗原不复制异源ori在允许或nonpermissive细胞中的预期更保守。我们推断，在体内观察到的乳头瘤病毒的严格的物种和组织特异性并不完全是由于病毒DNA复制的直接限制。相反，病毒基因表达的转录控制必须发挥主导作用。
We have shown that E1 and E2 proteins of human papillomavirus type 11 (HPV-11) were essential to support the replication of the homologous viral origin (ori) in a transient replication assay, similar to reports on bovine papillomavirus type 1 (BPV-1). Unexpectedly, matched or even mixed combinations of E1 and E2 proteins from HPV-11 or BPV-1 replicated either ori in human, monkey, and rodent cell lines of epithelial or fibroblastic lineage, albeit with varied efficiencies. Either set of viral proteins was also able to initiate replication of ori-containing plasmids from many other human and animal papillomaviruses. Thus the interactions among the cis elements and trans factors of papillomaviruses are more conserved than expected from the other members of the papovavirus family, simian virus 40 and polyomavirus, for which large tumor antigen does not replicate a heterologous ori in either permissive or nonpermissive cells. We infer that the stringent species and tissue specificities observed for papillomaviruses in vivo are not entirely due to direct restrictions on viral DNA replication. Rather, transcriptional control of viral gene expression must play a dominant role.