Phenotypic characterization of Sodalis praecaptivus sp nov., a close non-insect-associated member of the Sodalis-allied lineage of insect endosymbionts
Phenotypic characterization of Sodalis praecaptivus sp nov., a close non-insect-associated member of the Sodalis-allied lineage of insect endosymbionts
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DOI：
10.1099/ijs.0.000091
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发表时间：
2015-05-01
影响因子：
2.8
通讯作者：
Dale, Colin
中科院分区：
文献类型：
--
作者：
Chari, Abhishek;Oakeson, Kelly F.;Dale, Colin
从人类伤口中分离出的一种革兰氏染色阴性细菌，以前被发现与Sodalis glossinidius和Sodalis-allied Glade昆虫共生体的其他成员有着前所未有的密切关系。通过16 S rRNA基因序列分析和基因组比较，推测该菌株属于一个新种。生物化学和遗传分析支持这一建议，并表明，该生物体具有广泛的代谢特性，这是一个相对较大的基因库存的存在是一致的。在Sodalis相关分支的成员中，这是第一个具有足够代谢能力以维持在基本培养基中生长的代表。根据本研究的结果，我们建议将该微生物分类为一个新物种的代表，Sodalis praecaptivus sp. nov.（模式菌株HST = DSM 27494（T）= ATCC BAA-2554（T））。
A Gram-stain-negative bacterium, isolated from a human wound was previously found to share an unprecedentedly close relationship with Sodalis glossinidius and other members of the Sodalis-allied Glade of insect symbionts. This relationship was inferred from sequence analysis of the 16S rRNA gene and genomic comparisons and suggested the strain belonged to a novel species. Biochemical and genetic analyses supported this suggestion and demonstrated that the organism has a wide repertoire of metabolic properties, which is consistent with the presence of a relatively large gene inventory. Among members of the Sodalis-allied clade, this is the first representative that has sufficient metabolic capabilities to sustain growth in minimal media. On the basis of the results of this study, we propose that this organism be classified as a representative of a novel species, Sodalis praecaptivus sp. nov. (type strain HST = DSM 27494(T) = ATCC BAA-2554(T)).