Enhanced reactivity of peripheral blood immune cells to HSV-1 in primary achalasia
Enhanced reactivity of peripheral blood immune cells to HSV-1 in primary achalasia
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原发性贲门失弛缓症患者外周血免疫细胞对 HSV-1 的反应性增强
DOI：
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发表时间：
2010
影响因子：
1.9
通讯作者：
B. Johnston
中科院分区：
文献类型：
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作者：
K. Lau;C. Mccaughey;P. Coyle;L. Murray;B. Johnston
抽象目标。贲门失弛缓症是最具特征的食管运动障碍，但病因不明。病理学的特征是产生一氧化氮的神经元减少和肌间神经丛中存在对HSV-1病毒抗原有反应的活化T细胞炎性浸润。这些发现在正常对照中不存在。目前的研究比较了原发性贲门失弛缓症患者和正常对照者外周血单核细胞（PBMC）的反应性，以确定患者的PBMC是否对病毒表现出类似的高反应性。材料和方法。用151例原发性贲门失弛缓症患者和118例健康对照者的肝素化外周静脉血进行全血培养实验。在紫外线灭活HSV-1或条件细胞培养基存在下培养全血。通过使用Taqman®实时聚合酶链反应测量细胞因子基因干扰素-γ的表达来定量单核细胞对病毒抗原的反应性。数据表示为细胞因子倍数变化，其对应于抗原刺激的细胞与未刺激的细胞中产生的干扰素-γ信使RNA拷贝的比率。结果IFN-γ倍数变化病例组为61.33（20.54-217.00），对照组为49.67（10.05-157.05）。病例组和对照组之间的平均倍数变化差异为1.66倍（95%置信区间1.17-2.34，p = 0.004）。结论.这些结果表明，原发性贲门失弛缓症患者的PBMC显示出对HSV-1抗原的增强的免疫应答。这些数据表明，1型单纯疱疹病毒（HSV-1）或HSV-1样抗原部分对免疫细胞有持续刺激作用。
Abstract Objective. Achalasia is the best characterized oesophageal motor disorder but the etiology is unknown. The pathology is characterized by a decrease in nitric oxide-producing neurons and the presence of an activated T-cell inflammatory infiltrate in the myenteric plexus that are reactive to HSV-1 viral antigens. These findings are not present in normal controls. The current study compared the reactivity of peripheral blood mononuclear cells (PBMCs) between patients with primary achalasia and normal controls to determine if PBMCs of patients exhibit a similar heightened reactivity to the virus. Material and methods. Whole blood culture experiments were conducted with heparinized peripheral venous blood obtained from 151 patients with primary achalasia and 118 healthy controls. Whole blood was cultured in the presence of ultraviolet inactivated HSV-1 or conditioned cell culture media. Reactivity of mononuclear cells to viral antigens was quantified by measuring expression of the cytokine gene interferon-γ using Taqman® real-time polymerase chain reaction. Data are expressed as cytokine fold change corresponding to ratio of interferon-γ messenger RNA copies produced in antigen stimulated versus unstimulated cells. Results. The interferon-γ fold change was higher in cases 61.33 (20.54–217.00) than controls 49.67 (10.05–157.05). Mean fold change difference between cases and controls was 1.66 times (95% confidence interval 1.17–2.34, p = 0.004). Conclusions. These results indicate that the PBMCs of patients with primary achalasia show an enhanced immune response to HSV-1 antigens. The data suggest that there is persistent stimulation of immune cells by herpes simplex virus type 1 (HSV-1) or HSV-1 like antigen moieties.
γ 干扰素的产生似乎可以预测唇疱疹的复发时间。
DOI：
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发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Cunningham,AL;Merigan,TC
通讯作者：
Merigan,TC