Comparative transcriptome analysis on the synthesis pathway of honey bee (Apis mellifera) mandibular gland secretions.
Comparative transcriptome analysis on the synthesis pathway of honey bee (Apis mellifera) mandibular gland secretions.
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DOI：
10.1038/s41598-017-04879-z
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发表时间：
2017-07-03
影响因子：
4.6
通讯作者：
Hu F
中科院分区：
文献类型：
--
作者：
Wu Y;Zheng H;Corona M;Pirk C;Meng F;Zheng Y;Hu F
下颌腺分泌物具有重要的等级特异性功能，与蜜蜂的社会进化有关。为了深入了解这些种姓差异背后的分子结构，我们使用高通量RNA测序技术比较了来自蜂王、右蜂王工蜂（WQR）和无蜂王工蜂（WQL）的MG的基因表达模式。我们共鉴定了46个与MG中脂肪酸信息素的种姓特异性生物合成相关的候选基因，包括细胞色素P450（CYP450）家族成员和参与脂肪酸β-氧化和ω-氧化的基因。为了进一步鉴定参与MG分泌物生物合成的CYP 450 s基因，我们通过qPCR分析了转录组分析中最丰富表达的6种CYP 450基因在不同种姓、年龄、任务和组织中的表达水平。我们的分析表明，CYP 6AS 8和CYP 6AS 11分别是工蜂和蜂王MG中表达最丰富的CYP 450基因，与其他组织相比，它们在工蜂和蜂王的MG中选择性表达。这些结果表明，这些基因可能是负责关键的分叉羟基化过程中的生物合成途径。我们的研究有助于脂肪酸衍生的信息素在MG的生物合成的分子基础的描述。
Secretions from mandibular glands (MGs) have important caste-specific functions that are associated with the social evolution of honey bees. To gain insights into the molecular architecture underlying these caste differences, we compared the gene expression patterns of MGs from queens, queenright workers (WQRs) and queenless workers (WQLs) using high-throughput RNA-sequencing technology. In total, we identified 46 candidate genes associated with caste-specific biosynthesis of fatty acid pheromones in the MG, including members of cytochrome P450 (CYP450) family and genes involved in fatty acid β-oxidation and ω-oxidation. For further identification of the CYP450s genes involved in the biosynthesis of MG secretions, we analyzed by means of qPCR, the expression levels of six of the CYP450 genes most abundantly expressed in the transcriptome analysis across different castes, ages, tasks and tissues. Our analysis revealed that CYP6AS8 and CYP6AS11, the most abundantly expressed CYP450 genes in worker and queen MGs, respectively, are selectively expressed in the MGs of workers and queens compared to other tissues. These results suggest that these genes might be responsible for the critical bifurcated hydroxylation process in the biosynthesis pathway. Our study contributes to the description of the molecular basis for the biosynthesis of fatty acid-derived pheromones in the MGs.