Reduced T-Cell Thymic Export Reflected by sj-TREC in Patients with Coronary Artery Disease
Reduced T-Cell Thymic Export Reflected by sj-TREC in Patients with Coronary Artery Disease
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冠状动脉疾病患者中 sj-TREC 反映 T 细胞胸腺输出减少
DOI：
10.5551/jat.31575
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发表时间：
2016-01-01
影响因子：
4.4
通讯作者：
Liang, Chun
中科院分区：
文献类型：
--
作者：
Huang, Shuaibo;Ding, Ru;Liang, Chun
目的：免疫功能紊乱是动脉粥样硬化发生发展的重要机制之一。胸腺退化可导致免疫功能紊乱和T细胞亚群失衡。本研究旨在探讨冠心病（CAD）患者近期胸腺移出（RTE）功能是否受损。方法：检测CAD患者和年龄匹配的正常对照组T淋巴细胞信号联合T细胞受体切除环（sj-TREC）的含量。为了排除T细胞增殖对sj-TREC稀释度的潜在影响，使用单色多重定量PCR方法评估样品的端粒长度。根据Gensini评分（GS）（低，GS < 18;中等，GS 18-41;高，GS > 41）对患者进行分组。结果：不稳定型心绞痛患者、稳定型心绞痛患者和对照组每106个T淋巴细胞sj-TREC平均拷贝数分别为726+/-429，1213 +/-465，1795 +/-838（P
Aim: Immunologic dysfunction was recently found to be one of the most important mechanisms underlying the initiation and development of atherosclerosis. Thymus involution can contribute to immune disturbance and disequilibrium of T-cell subsets. This study aimed to explore whether recent thymic emigration (RTE) is impaired in patients with coronary artery disease (CAD).Methods: Content of signal-joint T cell receptor excision circles (sj-TREC) in T lymphocytes, a molecular marker of RTE, was assessed among CAD patients and age-matched controls. Monochrome multiplex quantitative PCR method was used to assess the samples' telomere length in order to exclude the potential influence of T cell proliferation on the dilution of sj-TREC. Patients were grouped according to Gensini score (GS) (low, GS < 18; intermediate, GS 18-41; high, GS > 41). Ordinary logistic regression models were used to determine potential risk factors for CAD and GS tertiles.Results: Average copy numbers of sj-TREC per 106 T lymphocytes among patients with unstable angina, stable angina, and controls were 726+/-429, 1213+/-465, and 1795+/-838, respectively (P