Stable and reproducible synthesis of gold nanorods for biomedical applications: a comprehensive study
Stable and reproducible synthesis of gold nanorods for biomedical applications: a comprehensive study
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DOI：
10.1049/iet-nbt.2016.0220
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发表时间：
2018-03-01
影响因子：
2.3
通讯作者：
Raza, Abida
中科院分区：
文献类型：
--
作者：
Awan, Uzma Azeem;Ali, Shaukat;Raza, Abida
金纳米棒 (GNR) 因其易于合成、可调的等离子体特性、毒性较小且易于检测而成为生物医学研究的理想选择，但其多样化的生物应用需要稳定的结构。尽管二十年来为可重复的各向异性结构合成做出了努力，但 GNR 生长过程中的动力学控制仍未实现。本研究试图了解合成具有所需长宽比的单分散、可重复且高度稳定的 GNR 的热力学和动力学参数。分析了各种生长参数和测定步骤对 GNR 简便且可重复合成的影响。通过基于颗粒不稳定性参数（PIP60 摄氏度）的紫外-可见光谱研究 GNR 的环境和生物胶体稳定性，碱性 pH 值会引发胶体不稳定性。 GNR 在较高盐浓度、生理和微酸性 pH 条件下保持稳定。 GNR 可在 0.001M 十六烷基三甲基溴化铵中保存 3 个月，而不会影响其稳定性。聚乙二醇化 GNR 在细胞介质溶液中非常稳定 (PIP
Gold nanorods (GNRs) are ideal choice in biomedical research due to their amenability of synthesis, tunable plasmonic properties, less toxicity and ease of detection but their diverse biological applications necessitate stable structure. Despite two decades' efforts made towards reproducible anisotropic structures synthesis, still the kinetic control during GNRs growth has not been achieved. This study is an attempt to apprehend thermodynamic and kinetic parameters for synthesising mono-disperse, reproducible and highly stable GNRs with desired aspect ratios. Effects of various growth parameters and assay steps on the facile and reproducible synthesis of GNRs are analysed. GNRs' environmental and biological colloidal stability is studied through UV-Vis spectroscopy based particle instability parameter (PIP60 degrees C) and alkaline pH can trigger colloidal instability. GNRs remain stable at higher salt concentration, physiological and slightly acidic pH. GNRs can be stored in 0.001M cetyltrimethylammonium bromide for 3 months without compromising their stability. PEGylated GNRs are quite stable in cellular media solution (PIP