The Stromal Cell-derived Factor-1 Expression Protected in Periodontal Tissues Damage during Occlusal Traumatism
The Stromal Cell-derived Factor-1 Expression Protected in Periodontal Tissues Damage during Occlusal Traumatism
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DOI：
10.2485/jhtb.30.63
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发表时间：
2021
影响因子：
0.4
通讯作者：
K. T. Goto;H. Kajiya;T. Tsutsumi;Munehisa Maeshiba;T. Tsuzuki;Kimiko Ohgi;M. Kawaguchi;J. Ohno;K. Okabe
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文献类型：
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作者：
K. T. Goto;H. Kajiya;T. Tsutsumi;Munehisa Maeshiba;T. Tsuzuki;Kimiko Ohgi;M. Kawaguchi;J. Ohno;K. Okabe
：过度咬合过程中的机械应力 (MS) 会导致牙槽硬线消除、骨吸收增强和牙齿脱落，从而导致咬合创伤。多项研究表明，MS 在牙周炎和正畸治疗的牙槽骨吸收过程中诱导细胞因子和趋化因子的表达。然而，咬合过度 MS 对维持牙槽骨代谢的影响仍不清楚。使用体内和体外过度闭塞模型，我们研究了 MS 对应激依赖性趋化因子和成骨细胞生成相关趋化因子表达之间关系的影响。在 6 周龄小鼠的体外模型中，MS 上调了牙周组织中基质细胞衍生因子 1 (SDF-1) 的表达，SDF-1 是 MS 后第 4 天骨髓干细胞中的一种自我更新趋化因子。相比之下，在 30 周龄小鼠的体内模型中，牙周组织中 MS 后第 1 天的 SDF-1 表达显着上调。在体内小鼠模型中，MS 在第 4 天诱导 PDL 根枝中 SDF-1 的表达以及牙髓和骨髓中 SDF-1 受体 CXCR4 的表达。 MS 上调 PDL 细胞中 SDF-1 的表达。 SDF-1 与 CXCR4 结合，在咬合创伤期间产生抗 MS 牙槽骨保护。
: Mechanical stress (MS) during hyperocclusion results in elimination of the alveolar hard line, enhancement of bone resorption, and shedding of a tooth, resulting in trauma of occlusion. Several studies have indicated that MS induces cytokine and chemokine expression during alveolar bone absorption in periodontitis and orthodontic treatment. However, it remains unknown regarding the effect of hyperocclusal MS on maintenance of alveolar bone metabolism. Using in vivo and in vitro hyperocclusion models, we investigated the effect of MS on relationships between the expression of stress-depend ent and osteoblastogenesis-associated chemokines. In the in vitro model with 6-weeks old mice, MS upregulated the expression of stromal cell-derived factor-1 (SDF-1) in periodontal tissues, a self-renewal chemokine in bone marrow stem cell day 4 after MS. In contrast, in the in vivo model with 30-weeks old mice, SDF-1 expression was significantly upregulated at day 1 after MS in periodontal tissues. MS induced the expression of SDF-1 in the PDL root branch and of the SDF-1 receptor CXCR4 in the dental pulp and bone marrow on day 4 in an in vivo mice model. MS upregulated the expression of SDF-1 in PDL cells. SDF-1 binds to CXCR4, resulting in MS-resistant alveolar bone protection during occlusal traumatism.