The cytotoxic effect of surgical glove powder particles on adult human vascular endothelial cell cultures: implications for clinical uses of tissue culture techniques.
The cytotoxic effect of surgical glove powder particles on adult human vascular endothelial cell cultures: implications for clinical uses of tissue culture techniques.
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手术手套粉末颗粒对成人血管内皮细胞培养物的细胞毒性作用：对组织培养技术临床应用的影响。
DOI：
10.1016/0022-4804(86)90163-0
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Rich,NM
中科院分区：
文献类型：
--
作者：
Sharefkin,JB;Fairchild,KD;Albus,RA;Cruess,DF;Rich,NM
血管假体(VP)的自体内皮细胞(EC)种植的临床应用需要可靠的EC收获方法，以便在医院或手术室环境中立即种植或原代培养。对原代培养失败的成人隐静脉内皮细胞(AHSVEC)中手套粉颗粒(GPP)的观察，使我们研究了外科GPP对培养的AHSVEC的影响。在培养的血管内皮细胞培养液中加入GPP后，细胞数呈剂量依赖性减少，细胞数减少50%所需的GPP平均浓度为1.5±0.8(SD)×104GPP/ml(N=10个实验)，相当于36μg手套粉/毫升的平均剂量。这种作用在加入GPP后24小时内出现，不是由于干扰EC的附着和铺展，也不是由于介质渗透压、pH、葡萄糖、电解质、钙或镁含量的变化。相反，这种效果似乎是由于在手套制造的最后橡胶硫化阶段添加了可过滤毒素，因为纯玉米淀粉颗粒和经环氧氯丙烷处理的纯玉米淀粉不会阻止菌体生长，而与直接从手套中提取的GPP孵育的0.2GPP培养基滤液是致命的。我们的结论是，GPP中可过滤的细胞毒性物质可能是VP EC种植失败的一个可避免的原因，并可能影响手术伤口的愈合。
Clinical use of autogenous endothelial cell (EC) seeding of vascular prostheses (VP) would require reliable methods for EC harvest for immediate seeding or primary culture in a hospital or operating room setting. Observation of glove powder particles (GPP) in failed primary adult human saphenous vein EC (AHSVEC) cultures led us to study the effect of surgical GPP on cultured AHSVEC. Addition of GPP to the culture medium of growing ASHVEC cultures reduced the cell counts in a dose-dependent fashion; the mean concentration of GPP required to produce a >50% decrease in cell number was 1.5 ± 0.8 (SD) × 104GPP/ml (N= 10 experiments), equivalent to a mean dose of 36 μg glove powder per milliliter. The effect was seen within 24 hr of addition of GPP and was not due to interference with EC attachment and spreading or to changes in medium osmolality, pH, glucose, electrolyte, Ca2+, or Mg2+content. Instead, the effect appeared to be due to a filterable toxin added during the final rubber-vulcanizing stage of glove manufacture, since pure cornstarch particles and epichlorhydrin-treated pure cornstarch did not prevent culture growth, whereas 0.2 μm filtrates of medium incubated with GPP taken directly from gloves were lethal. We conclude that filterable cytotoxic substances from GPP may be an avoidable cause of failure in EC seeding of VP, and may affect surgical wound healing as well.