Carbon Dots with Absorption Red-Shifting for Two-Photon Fluorescence Imaging of Tumor Tissue pH and Synergistic Phototherapy
Carbon Dots with Absorption Red-Shifting for Two-Photon Fluorescence Imaging of Tumor Tissue pH and Synergistic Phototherapy
复制标题
具有吸收红移的碳点用于肿瘤组织 pH 值的双光子荧光成像和协同光疗
DOI：
10.1021/acsami.1c08076
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发表时间：
2021-07-21
影响因子：
9.5
通讯作者：
Zhao, Shulin
中科院分区：
文献类型：
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作者：
Bai, Yulong;Zhao, Jingjin;Zhao, Shulin
光疗法作为一种新型的肿瘤治疗方法具有巨大的潜力，高活性光敏剂和光热剂的开发引起了人们的广泛关注。以赖氨酸、邻苯二胺和硫酸为原料，采用水热法制备了具有吸收红移效应的硫氮共掺杂碳量子点（S，N-CD）。S、N-CD的近红外（NIR）吸收特性导致双光子（TP）发射，其已分别用于溶酶体和肿瘤组织pH的TP荧光成像以及肿瘤光疗过程中细胞凋亡的实时监测。所获得的杂原子共掺杂的CD不仅可以用作NIR成像探针，而且可以用作有效的光动力疗法/光热疗法（PDT/PTT）治疗剂。比较了不同杂原子掺杂的CD在肿瘤治疗中的效率。结果表明，S，N-CD比N掺杂CD具有更高的治疗效率，O-1（2）的产生效率为27%，光热转换效率达到34.4%。该研究为碳基纳米药物的合成提供了新的见解，用于协同光疗和肿瘤的准确诊断。
Phototherapy exhibits significant potential as a novel tumor treatment method, and the development of highly active photosensitizers and photothermal agents has drawn considerable attention. In this work, S and N atom co-doped carbon dots (S,N-CDs) with an absorption redshift effect were prepared by hydrothermal synthesis with lysine, o-phenylenediamine, and sulfuric acid as raw materials. The near-infrared (NIR) absorption features of the S,N-CDs resulted in two-photon (TP) emission, which has been used in TP fluorescence imaging of lysosomes and tumor tissue pH and real-time monitoring of apoptosis during tumor phototherapy, respectively. The obtained heteroatom co-doped CDs can be used not only as an NIR imaging probe but also as an effective photodynamic therapy/photothermal therapy (PDT/PTT) therapeutic agent. The efficiencies of different heteroatom-doped CDs in tumor treatment were compared. It was found that the S,N-CDs showed higher therapeutic efficiency than N-doped CDs, the efficiency of producing O-1(2) was 27%, and the photothermal conversion efficiency reached 34.4%. The study provides new insight into the synthesis of carbon-based nanodrugs for synergistic phototherapy and accurate diagnosis of tumors.