Chromaffin vesicle function in intact cells.
Chromaffin vesicle function in intact cells.
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嗜铬小泡在完整细胞中发挥功能。
DOI：
10.1111/j.1749-6632.1987.tb27202.x
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发表时间：
1987
影响因子：
5.2
通讯作者：
Korner,M
中科院分区：
文献类型：
--
作者：
Kirshner,N;Corcoran,JJ;Caughey,B;Korner,M
通过对分离的嗜铬小泡的研究和对完整动物、分离的灌流腺和肾上腺髓质细胞培养的诱发分泌的研究，阐明了染色质和小泡在肾上腺髓质细胞中的生理作用。I4嗜铬小泡含有高浓度的儿茶酚胺和三磷酸腺苷，并含有催化多巴胺氧化成去甲肾上腺素的多巴胺/？羟基酶。它们直接参与肾上腺素和去甲肾上腺素的胞吐分泌，囊泡膜上配备了一种依赖于ATP的运输系统，用于在高浓度梯度下将儿茶酚胺转移到囊泡中。尽管去甲肾上腺素和肾上腺素形成的个别反应已经被很好地描述，但在进行本文报道的研究时，关于这一途径的综合功能的信息很少。分离的染色质小泡可摄取三磷酸腺苷，但对三磷酸腺苷在完整细胞染色质和小泡中的积累及其周转知之甚少。本文报道了在完整的牛肾上腺髓质细胞培养中，儿茶酚胺通过色度和囊泡的流量和ATP代谢的研究。
The physiological role of chroma& vesicles in adrenal medullary cells has been elucidated through studies of the isolated chromaffin vesicles and through studies of evoked secretion employing intact animals, isolated perfused glands, and adrenal medullary cell cultures. I4 The chromafin vesicles contain high concentrations of catecholamines and ATP and they contain the enzyme dopamine/.?-hydroxylase, which catalyzes the oxidation of dopamine to norepinephrine. They are directly involved in the exocytotic secretion of epinephrine and norepinephrine, and the vesicle membranes are equipped with an ATP-dependent transport system for translocating catechol-amines into the vesicles against a high concentration gradient. Although the individual reactions in the formation of norepinephrine and epinephrine have been well characterized, there was only little information on the integrated function of this pathway at the time the studies reported here were undertaken. Isolated chromatlin vesicles can take up ATP, but little was known about the accumulation of ATP or its turnover in chroma& vesicles of intact cells. Studies in which we have examined the flux of catecholamines through chroma& vesicles5 and ATP metabolism in cultures of intact bovine adrenal medullary cells6.'are reported here.