RECEPTIVE-FIELDS OF CRICKET GIANT INTERNEURONES ARE RELATED TO THEIR DENDRITIC STRUCTURE
RECEPTIVE-FIELDS OF CRICKET GIANT INTERNEURONES ARE RELATED TO THEIR DENDRITIC STRUCTURE
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DOI：
10.1113/jphysiol.1984.sp015312
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发表时间：
1984-01-01
影响因子：
5.5
通讯作者：
MURPHEY, RK
中科院分区：
文献类型：
--
作者：
BACON, JP;MURPHEY, RK
采用细胞内记录技术和染色注射技术，研究了蟋蟀中枢-巨中枢神经系统传入投射与中间神经元树突之间的结构关系。通过在剪断的头发末端放置一个记录移液管，并利用移液管的运动来表征受体的方向性，研究了宫颈丝状毛下感觉神经元的生理学。大多数丝状受体可归为4种主要类型之一。每种类型对不同的风向都很敏感，并且局限于尾蚴的特定区域。用CoCl染色显示了各种感觉细胞的末端分枝的位置。将一个充满染料的移液管置于剪下的头发上，对单细胞进行可靠的染色。每种生理受体类型都分布在中枢神经系统的不同区域。神经堆根据风向进行功能划分。在这种传入投射的背景下，对3个确定的中间神经元的树突进行了检查。这些神经元中的每一个都在神经堆对应不同风向的区域有树突。通过在确定的中间神经元中寻找统一的突触电位，可以显示初级传入神经元和中间神经元的解剖重叠与突触连接的存在之间存在很强的相关性。当没有重叠时，没有观察到突触电位。中间神经元的总体兴奋性感受野可以通过检查其树突结构来预测。在本研究中发现的3个确定的中间神经元中的每一个都有一个定向反应，该反应与基于其解剖结构预测的反应相匹配。
The structural relationship between the afferent projection and the dendrites of the interneurons was examined in the cercal-to-giant interneuron system of the cricket using intracellular recording and dye injection techniques. The physiology of the sensory neurons beneath the cercal filiform hairs was investigated by placing a recording pipette over the end of a cut hair and using movements of the pipette to characterize the directionality of the receptor. Most of the filiform receptors could be classified as belonging to 1 of 4 major types. Each type is sensitive to a different wind direction and is confined to particular regions of the cercus. The location of the terminal arborizations of each type of sensory cell was revealed by staining with CoCl. single cells were stained reliably by placing a dye-filled pipette over a cut hair. Each physiological receptor type arborizes in a different region of the CNS. The neuropile is functionally divided according to wind direction. The dendrites of 3 identified interneurons were examined in the context of this afferent projection. Each of these neurons has dendrites in regions of neuropile corresponding to different wind directions. By searching for unitary synaptic potentials in identified interneurons, it was possible to show a strong correlation between anatomical overlap of primary afferent and interneuron and the existence of a synaptic connection. When there was no overlap, no synaptic potentials were seen. The over-all excitatory receptive field of an interneuron could be predicted by examining its dendritic structure. Each of the 3 identified interneurons examined in this study was found to have a directional response that matched the response predicted on the basis of its anatomy.