MiR-139-5p inhibits the proliferation of gastric cancer cells by targeting Regulation of Nuclear Pre-mRNA Domain Containing 1B
MiR-139-5p inhibits the proliferation of gastric cancer cells by targeting Regulation of Nuclear Pre-mRNA Domain Containing 1B
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MiR-139-5p通过靶向调节含有1B的核前体mRNA结构域抑制胃癌细胞的增殖
DOI：
10.1016/j.bbrc.2020.04.067
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发表时间：
2020-06-25
影响因子：
3.1
通讯作者：
Chen Lin
中科院分区：
文献类型：
--
作者：
Liang Wenquan;Xi Hongqing;Chen Lin
核前mRNA结构域1B（RPRD 1B）的调控是近年来肿瘤学领域的研究热点。胃癌（GC）中miRNAs与RPRD 1B的关系尚未得到充分报道。本研究旨在筛选RPRD 1B靶向的miRNAs，并探讨其在胃癌细胞中的调控机制。采用定量RT-PCR和原位杂交技术检测胃癌组织中miRNA的表达。采用集落形成法、EdU细胞增殖实验和流式细胞术分析细胞周期。数据库辅助的基因表达分析显示，RPRD 1B在GC中被miRNA-139- 5 p靶向和调节。miRNA-139- 5 p在胃癌组织中的表达高于正常组织，并与胃癌的肿瘤大小、病理分期和无病生存期显著相关（p < 0.05）。miRNA-139- 5 p调控胃癌细胞增殖并影响G1期向S期的转变。它与RPRD 1B的3 'UTR的2013-2019位点明确结合，并负调控RPRD 1B表达。我们证明了miR-139- 5 p调节GC细胞增殖的能力依赖于RPRD 1B。这一过程伴随着Cyclin D1蛋白表达的变化。我们在胃癌中建立了miR-139- 5 p/RPRD 1B/肿瘤增殖轴，这可能成为胃癌的新生物标志物和药物靶点。(C)2020爱思唯尔公司All rights reserved.
Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B) has been of great interest in the field of oncology in recent years. The relationship between miRNAs and RPRD1B in gastric cancer (GC) has not been adequately reported. This study was designed to screen RPRD1B-targeted miRNAs and investigate its regulatory mechanism in GC cells. Quantitative RT-PCR and in situ hybridization were used to detect miRNA expression in GC tissues. Colony formation, EdU cell proliferation assay, and flow cytometry were used to analyze the cell cycle. Database-assisted gene expression analysis revealed that RPRD1B was targeted and regulated by miRNA-139-5p in GC. miRNA-139-5p expression was higher in GC tissue than in normal tissues and significantly correlated with tumor size, pathological stage, and disease-free survival of GC (p < 0.05). MiRNA-139-5p regulates GC cell proliferation and affects the transition from G1 to S phase. It binds explicitly to the 2013-2019 sites of the 3'UTR of RPRD1B and negatively regulates RPRD1B expression. We demonstrated that the ability of miR-139-5p to regulate GC cell proliferation depends on RPRD1B. This process is accompanied by changes in Cyclin D1 protein expression. We established a miR-139-5p/RPRD1B/tumor proliferation axis in GC, which may serve as novel biomarkers and drug targets for GC. (C) 2020 Elsevier Inc. All rights reserved.