Sodium 2-propenyl thiosulfate derived from garlic induces phase II detoxification enzymes in rat hepatoma H4IIE cells
Sodium 2-propenyl thiosulfate derived from garlic induces phase II detoxification enzymes in rat hepatoma H4IIE cells
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DOI：
10.1016/j.nutres.2010.06.007
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发表时间：
2010-06-01
影响因子：
4.5
通讯作者：
Yamato, Osamu
中科院分区：
文献类型：
--
作者：
Chang, Hye-Sook;Ko, Miyan;Yamato, Osamu
有证据表明洋葱和大蒜可以预防人类癌症。有人认为，这种影响部分是由于葱类蔬菜中的有机硫化合物，这些物质通过诱导第二阶段解毒酶发挥作用。在这里，我们假设，烷（烯）基硫代硫酸盐，正丙基硫代硫酸钠（NPTS），2-丙烯基硫代硫酸钠（2 PTS），这是确定在洋葱和大蒜，分别可能会诱导II相酶。因此，将大鼠肝癌细胞（H4 IIE）与1至100 μ mol/L的NPTS或2 PTS在37 ℃下培养48小时;并研究H4 IIE细胞中II相酶的活性和信使RNA（mRNA）表达水平。二烯丙基三硫化物和叔丁基对苯二酚（称为11相诱导剂）的作用也作为阳性对照进行了检查，并与NPTS和2 PTS的反应进行了比较。醌还原酶（QR）活性和QR和环氧化物水解酶1的mRNA表达水平显著增加（P <0.05 - 0.005）。特别地，QR活性在相对低浓度的2 PTS（10 μ mol/L）下增加。然而，谷胱甘肽S-转移酶活性和谷胱甘肽S-转移酶A5和尿苷二磷酸葡萄糖醛酸转移酶1A 1的mRNA表达水平没有改变2 PTS。相反，NPTS不影响这些II相酶的活性和mRNA表达水平。这些结果表明，2 PTS可以诱导II相酶，其诱导效果与二烯丙基三硫和叔丁基对苯二酚相当或上级。(C)2010年爱思唯尔公司All rights reserved.
There is evidence that onions and garlic protect against cancer in humans. It has been suggested that this effect is partly due to the organosulfur compounds in Allium vegetables and that these substances act through induction of phase II detoxification enzymes. Here, we hypothesized that alk (en)yl thiosulfates, sodium n-propyl thiosulfate (NPTS), and sodium 2-propenyl thiosulfate (2PTS), which were identified in onions and garlic, respectively, may induce phase II enzymes. Therefore, rat hepatoma cells (H4IIE) were cultured with 1 to 100 mu mol/L of NPTS or 2PTS for 48 hours at 37 degrees C; and the activities and messenger RNA (mRNA) expression levels of phase II enzymes in H4IIE cells were investigated. The effects of diallyl trisulfide and tert-butylhydroquinone, known as phase 11 inducers, were also examined as positive controls and compared with the responses of NPTS and 2PTS. Quinone reductase (QR) activity and mRNA expression levels of QR and epoxide hydrolase 1 were significantly increased by 2PTS (P < .05-.005). In particular, QR activity was increased at a relatively low concentration of 2PTS (10 mu mol/L). However, glutathione S-transferase activity and mRNA expression levels of glutathione S-transferase A5 and uridine diphosphate glucuronosyl transferase 1A1 were not changed by 2PTS. In contrast, NPTS did not affect the activities and mRNA expression levels of these phase II enzymes. These results show that 2PTS can induce phase II enzymes, and its inductive effect is comparable or superior to that of diallyl trisulfide and tert-butylhydroquinone. (C) 2010 Elsevier Inc. All rights reserved.