Adenovirus type-2 infection of human keratinocytes: viral expression dependent upon the state of cellular maturation.
Adenovirus type-2 infection of human keratinocytes: viral expression dependent upon the state of cellular maturation.
复制标题
人类角质形成细胞的 2 型腺病毒感染：病毒表达取决于细胞成熟状态。
DOI：
10.1016/0042-6822(81)90015-5
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发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
Taichman,LB
中科院分区：
文献类型：
--
作者：
Laporta,RF;Taichman,LB
我们已经证明，腺病毒2型（AD-2）将有效地感染培养的人角质形成细胞，外胚层来源的细胞。在感染过程中，在基底上细胞中观察到细胞病变效应，而大多数基底细胞似乎不含病毒。用单独的基底细胞培养物和悬浮感染的细胞培养物进行的实验表明，基底细胞缺乏反应不是对感染的抗性的结果。显然，生产性反应在基底角质形成细胞中被阻断，但在一些未知的成熟事件之后，随后产生完全允许的和成熟的感染性颗粒。免疫荧光分析表明，阻断基底细胞中的病毒表达发生在T抗原合成之前。这些结果暗示Ad-2的表达依赖于角质形成细胞中细胞分化的状态。这个系统应该被证明是一个有价值的工具，在研究分化以及潜伏感染的性质。
We have demonstrated that adenovirus type-2 (AD-2) will productively infect cultured human keratinocytes, an ectodermally derived cell. During infection cytopathic effects are observed in suprabasal cells while most basal cells appear to be free of virus. Experiments with cultures of basal cells alone and with cells infected in suspension indicate that this lack of response of the basal cells is not a result of resistance to infection. Apparently the productive response is blocked in basal keratinocytes, but after some unknown maturational events ensue fully permissive and mature infectious particles are produced. Immunofluorescence analysis suggests that blockage of viral expression in the basal cells occurs prior to T-antigen synthesis. These results imply that expression of Ad-2 is dependent upon the state of cellular differentiation in keratinocytes. This system should prove to be a valuable tool in the study of differentiation as well as the nature of latent infection.