Folic Acid-Conjugated, SERS-Labeled Silver Nanotriangles for Multimodal Detection and Targeted Photothermal Treatment on Human Ovarian Cancer Cells
Folic Acid-Conjugated, SERS-Labeled Silver Nanotriangles for Multimodal Detection and Targeted Photothermal Treatment on Human Ovarian Cancer Cells
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DOI：
10.1021/mp400300m
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发表时间：
2014-02-01
影响因子：
4.9
通讯作者：
Astilean, Simion
中科院分区：
文献类型：
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作者：
Boca-Farcau, Sanda;Potara, Monica;Astilean, Simion
癌症治疗剂的有效性在于它能在不损害附近健康组织的情况下减少和消除肿瘤。纳米颗粒外周结合靶向部分通过位点特异性提供治疗的重大改进。在这项研究中，我们通过靶向NIH:OVCAR-3人卵巢癌细胞系的叶酸受体来证明这种方法。本文采用壳聚糖（生物）聚合物生物相容性、对氨基噻吩（pATP）拉曼报告分子标记、叶酸偶联的银纳米三角形。通过局部表面等离子体共振（LSPR）、zeta电位和表面增强拉曼散射（SERS）测量来研究纳米粒子的共轭和有效标记。共轭粒子被证明在水和细胞介质中高度稳定。通过暗场显微镜和细胞内粒子的散射光谱，证实了共轭纳米粒子被人卵巢癌细胞靶向摄取。比较研究表明，与类似的裸纳米颗粒相比，共轭纳米颗粒具有特异性内化。此外，粒子的SERS特性在细胞内被证明是高度保守的。用连续波-近红外（cu - nir）激光与其等离子体带共振照射纳米颗粒处理的细胞进行靶向癌细胞治疗，证明了有效的治疗反应。通过将多模态光学成像和SERS检测的优势与热疗能力结合起来，这些纳米颗粒可以代表个性化医疗的真正候选物。
The effectiveness of a therapeutic agent for cancer stands in its ability to reduce and eliminate tumors without harming the healthy tissue nearby. Nanoparticles peripherally conjugated with targeting moieties offer major improvements in therapeutics through site specificity. In this study we demonstrate this approach by targeting the folate receptor of NIH:OVCAR-3 human ovary cancer cell line. Herein we used silver nanotriangles which were biocompatibilized with chitosan (bio)polymer, labeled with para-aminothiophenol (pATP) Raman reporter molecule, and conjugated with folic acid. The nanoparticles conjugation and efficient labeling was investigated by localized surface plasmon resonance (LSPR), zeta potential, and surface-enhanced Raman scattering (SERS) measurements. Conjugated particles were proven to be highly stable in aqueous and cellular medium. The targeted uptake of conjugated nanoparticles by human ovary cancer cells was confirmed by dark field microscopy and scattering spectra of the particles inside cells. Comparative studies revealed specific internalization of the conjugated nanoparticles in comparison with similar bare nanoparticles. Moreover, the SERS identity of the particles was proven to be highly conserved inside cells. Targeted cancer cell treatment conducted by irradiating the nanoparticle-treated cells with a continuous wave-nearinfrared (cw-NIR) laser in resonance with their plasmonic band proved an efficient therapeutic response. By integrating the advantages of multimodal optical imaging and SERS detection with hyperthermia capabilities through site specificity, these nanoparticles can represent a real candidate for personalized medicine.