Poly(A)-containing messenger ribonucleoprotein complexes from sea urchin eggs and embryos: polypeptides associated with native and UV-crosslinked mRNPs.
Poly(A)-containing messenger ribonucleoprotein complexes from sea urchin eggs and embryos: polypeptides associated with native and UV-crosslinked mRNPs.
复制标题
来自海胆卵和胚胎的含有 Poly(A) 的信使核糖核蛋白复合物：与天然和 UV 交联的 mRNA 相关的多肽。
DOI：
10.1111/j.1432-0436.1983.tb01296.x
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Moon,RT
中科院分区：
文献类型：
--
作者：
Moon,RT
海胆卵的受精导致储存的信息快速募集到多核糖体中。海胆卵的翻译控制是否由与储存信息相关的大分子介导尚不清楚，因为信使核糖核蛋白复合物（mRNPs）的制备在兔网织网细胞裂解液中的蛋白质合成中具有活性。为了便于mRNPs的研究，采用寡聚（dT）‐纤维素层析法从紫圆海胆卵和胚胎中纯化含有聚(A)‐的mRNPs。从卵中纯化的非多核糖体mRNPs在蔗糖中的沉降与未纯化的mRNPs相似，在甲硝唑胺中的峰值浮力密度为1.22 g/cm3，在戊二醛固定后的cs2so4中的峰值浮力密度为1.42 g/cm3，而未固定的峰值浮力密度为1.46 g/cm3。来自卵和受精卵的非多核糖体mRNPs在十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶上含有5-10个主要蛋白，以及许多次要蛋白带。紫外光照射活体海胆卵产生交联mRNPs，其多肽模式与非交联mRNPs相似。与胚胎多核糖体mRNPs相关的多肽也与存在于非多核糖体mRNPs中的多肽在质量上相似，尽管化学计量差异可能存在。
Fertilization of sea urchin eggs results in the rapid recruitment of stored messages into polyribosomes. Whether translational control in sea urchin eggs is mediated by macromolecules associated with the stored messages remains unknown, since preparations of messenger ribonucleoprotein complexes (mRNPs) were active in protein synthesis in a rabbit reticulocyte lysate. To facilitate the study of mRNPs, chromatography on oligo(dT)‐cellulose was used to purify poly(A)‐containing mRNPs from eggs and embryos of the sea urchinStrongylocentrotus purpuratus. Nonpolyribosomal mRNPs purified from eggs had a similar sedimentation in sucrose to unpurified mRNPs, a peak buoyant density in metrizamide of 1.22 g/cm3, and peak buoyant densities in Cs2SO4in 1.42 g/cm3after fixation with glutaraldehyde and 1.46 g/cm3without fixation. Nonpolyribosomal mRNPs from eggs and zygotes contained 5–10 major proteins on sodium dodecylsulfate (SDS) polyacrylamide gels, and numerous minor bands. UV‐irradiation of living eggs of the sea urchinArbacia punctulataproduced cross‐linked mRNPs which contained a similar pattern of polypeptides to noncross‐linked mRNPs. The polypeptides associated with embyronic polyribosomal mRNPs were also qualitatively similar to those present in nonpolyribosomal mRNPs, although stoichiometric differences may exist.