Specific VEGF sequestering and release using peptide-functionalized hydrogel microspheres.
Specific VEGF sequestering and release using peptide-functionalized hydrogel microspheres.
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DOI：
10.1016/j.biomaterials.2012.01.032
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发表时间：
2012-04
期刊：
影响因子：
14
通讯作者：
Murphy, William L.
中科院分区：
文献类型：
--
作者：
Impellitteri, Nicholas A.;Toepke, Michael W.;Levengood, Sheeny K. Lan;Murphy, William L.
生长因子信号在组织发育、维护和修复等调节过程中发挥着重要作用。细胞外基质成分的基因表达水平、扩散、降解和隔离都在调节细胞微环境内生长因子的浓度中发挥作用。在此，我们描述了水凝胶微球的合成和表征，该水凝胶微球模拟天然细胞外基质在溶液中可逆地结合血管内皮生长因子（VEGF）的能力。将源自 VEGF 受体 2 (VEGFR2) 的肽配体共价掺入水凝胶微球中，以实现结合亲和力和特异性。除了能够以可控的方式结合和释放 VEGF 之外，微球还被证明可以影响人脐静脉内皮细胞 (HUVEC) 的增殖。由此产生的微球可以实现特异性上调或下调细胞微环境中生长因子信号传导的新策略。
Growth factor signaling plays an essential role in regulating processes such as tissue development, maintenance, and repair. Gene expression levels, diffusion, degradation, and sequestration by extracellular matrix components all play a role in regulating the concentration of growth factors within the cellular microenvironment. Herein, we describe the synthesis and characterization of hydrogel microspheres that mimic the ability of the native extracellular matrix to reversibly bind vascular endothelial growth factor (VEGF) out of solution. A peptide ligand derived from the VEGF receptor 2 (VEGFR2) was covalently incorporated into the hydrogel microspheres in order to achieve binding affinity and specificity. In addition to being able to both bind and release VEGF in a controllable manner, the microspheres were also shown to affect human umbilical vein endothelial cell (HUVEC) proliferation. The resulting microspheres may enable new strategies to specifically upregulate or downregulate growth factor signaling in the cellular microenvironment.
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影响因子：
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