Corticotropin-releasing hormone messenger RNA distribution and stress-induced activation in the thalamus
Corticotropin-releasing hormone messenger RNA distribution and stress-induced activation in the thalamus
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DOI：
10.1016/s0306-4522(01)00239-1
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Kalin, NH
中科院分区：
文献类型：
--
作者：
Hsu, DT;Lombardo, KA;Kalin, NH
促肾上腺皮质激素释放激素在介导应激反应中起关键作用。假设调节压力的大脑回路包括丘脑，丘脑在将感觉信息分配到皮层和皮层下结构中起着关键作用。原位杂交显示神经元含有促肾上腺皮质激素释放激素信使RNA在丘脑后核群和中央内侧核的丘脑，与腹后内侧核（小细胞部分）的接口。这些区域是感兴趣的，因为它们处理躯体感觉和内脏信息。在第一个实验中，急性应激对丘脑促肾上腺皮质激素释放激素信使RNA水平的影响进行了评估。束缚1h并于1h后处死的大鼠，发现丘脑后核群促肾上腺皮质激素释放激素信使RNA增加。在第二个实验中，这些变化的时间过程进行了检查，在该实验中，大鼠被立即或3小时后的约束。虽然没有变化发生在丘脑后立即限制，3小时后限制，促肾上腺皮质激素释放激素信使RNA的增加发生在丘脑后核组和中央内侧-腹后内侧核（小细胞部分）的丘脑。一个不同的模式的激活，观察到在下丘脑室旁核与促肾上腺皮质激素释放激素信使RNA增加后立即限制，但不是I或3小时后。除了应激诱导的变化，一个显着的减少基线丘脑促肾上腺皮质激素释放激素信使RNA观察到从1000至1300小时。这些结果表明，丘脑含有的促肾上腺皮质激素释放激素信使RNA的限制应激后增加，表明丘脑促肾上腺皮质激素释放激素系统在应激反应中的作用。应激诱导的丘脑促肾上腺皮质激素释放激素信使RNA表达的变化似乎受到不同的调节比下丘脑室旁核，并可能受到昼夜机制的影响。(C)2001年IBRO。由爱思唯尔科技有限公司出版。保留所有权利。
Corticotropin-releasing hormone plays a critical role in mediating the stress response. Brain circuits hypothesized to mediate stress include the thalamus, which plays a pivotal role in distributing sensory information to cortical and subcortical structures. In situ hybridization revealed neurons containing corticotropin-releasing hormone messenger RNA in the posterior thalamic nuclear group and the central medial nucleus of the thalamus, which interfaces with the ventral posteromedial nucleus (parvicellular part). These regions are of interest because they process somatosensory and visceral information. In the first experiment, the effect of acute stress on thalamic corticotropin-releasing hormone messenger RNA levels was assessed. Rats restrained for 1 h and killed 1 h later were found to have increased corticotropin-releasing hormone messenger RNA in the posterior thalamic nuclear group. The time course of these changes was examined in a second experiment in which rats were killed immediately or 3 h after restraint. While no changes occurred in the thalamus immediately after restraint, 3 h after restraint, increases in corticotropin-releasing hormone messenger RNA occurred in both the posterior thalamic nuclear group and the central medial-ventral posteromedial nucleus (parvicellular part) of the thalamus. A different pattern of activation was observed in the paraventricular nucleus of the hypothalamus with increased corticotropin-releasing hormone messenger RNA immediately after restraint, but not I or 3 h later. In addition to the stress-induced changes, a prominent decrease in baseline thalamic corticotropin-releasing hormone messenger RNA was observed from 1000 to 1300 h.These results show that the thalamus contains corticotropin-releasing hormone messenger RNA that increases after restraint stress, indicating a role for thalamic corticotropin-releasing hormone systems in the stress response. Stress-induced changes in thalamic corticotropin-releasing hormone messenger RNA expression appears to be regulated differently than that in the paraventricular nucleus of the hypothalamus, and may be influenced by diurnal mechanisms. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.