Tracking Single Cells Motility on Different Substrates.
Tracking Single Cells Motility on Different Substrates.
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DOI：
10.3390/mps3030056
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发表时间：
2020-08-04
影响因子：
2.4
通讯作者：
Chung BM
中科院分区：
文献类型：
--
作者：
Sharma P;Lam VK;Raub CB;Chung BM
运动性是细胞的关键特性，是几种生理过程所需的，包括胚胎发育、轴突引导、组织再生、原肠胚形成、免疫应答和癌症转移。因此，检查细胞运动性的能力，特别是在单细胞水平上，对于理解各种生物过程是重要的。目前有几种不同的测定法可用于检查细胞运动性。然而，在单细胞水平上研究细胞运动性可能是昂贵的和/或具有挑战性的。在这里，我们描述了一种方法，跟踪随机细胞运动在不同的基板，如玻璃，组织培养聚苯乙烯，和I型胶原蛋白水凝胶，它可以被修改，以产生不同的胶原蛋白网络的微观结构。在这项研究中，我们使用CytoSMARTTM系统（Lonza Group，巴塞尔，瑞士）跟踪MDA-MB-231乳腺癌细胞进行活细胞成像，并使用ImageJ和wrMTrck插件评估平均细胞迁移速度。我们的成本效益和易于使用的方法允许研究细胞运动在一个单一的细胞水平上的不同程度的刚度和不同的组成不同的基板。此过程可以通过简单的设置以高度可访问的方式成功执行。
Motility is a key property of a cell, required for several physiological processes, including embryonic development, axon guidance, tissue regeneration, gastrulation, immune response, and cancer metastasis. Therefore, the ability to examine cell motility, especially at a single cell level, is important for understanding various biological processes. Several different assays are currently available to examine cell motility. However, studying cell motility at a single cell level can be costly and/or challenging. Here, we describe a method of tracking random cell motility on different substrates such as glass, tissue-culture polystyrene, and type I collagen hydrogels, which can be modified to generate different collagen network microstructures. In this study we tracked MDA-MB-231 breast cancer cells using The CytoSMARTTM System (Lonza Group, Basel, Switzerland) for live cell imaging and assessed the average cell migration speed using ImageJ and wrMTrck plugin. Our cost-effective and easy-to-use method allows studying cell motility at a single cell level on different substrates with varying degrees of stiffness and varied compositions. This procedure can be successfully performed in a highly accessible manner with a simple setup.