Dynamic and social behaviors of human pluripotent stem cells.
Dynamic and social behaviors of human pluripotent stem cells.
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DOI：
10.1038/srep14209
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发表时间：
2015-09-18
影响因子：
4.6
通讯作者：
Reijo Pera RA
中科院分区：
文献类型：
--
作者：
Phadnis SM;Loewke NO;Dimov IK;Pai S;Amwake CE;Solgaard O;Baer TM;Chen B;Reijo Pera RA
人类多能干细胞（hPSC）可以自我更新或分化为不同的细胞类型，从而为基础和临床应用提供了平台。然而，多能干细胞群体是异质的，并且在单细胞水平上的功能特性记录不多，导致分化效率低下以及关于再现性和安全性的担忧。在这里，我们使用非侵入性延时成像来连续检查hPSC的维持和分化，并预测细胞活力和命运。我们记录了动态行为和社会互动，前瞻性地区分hPSC的生存，自我更新和分化。结果突出了E-钙粘蛋白不仅对于细胞-细胞接触而且对于hPSC的克隆繁殖的分子作用。结果表明，使用连续的延时成像可以区分细胞的异质性方面的多能性，以及一个子集的核型异常的动态特性进行了监测。
Human pluripotent stem cells (hPSCs) can self-renew or differentiate to diverse cell types, thus providing a platform for basic and clinical applications. However, pluripotent stem cell populations are heterogeneous and functional properties at the single cell level are poorly documented leading to inefficiencies in differentiation and concerns regarding reproducibility and safety. Here, we use non-invasive time-lapse imaging to continuously examine hPSC maintenance and differentiation and to predict cell viability and fate. We document dynamic behaviors and social interactions that prospectively distinguish hPSC survival, self-renewal, and differentiation. Results highlight the molecular role of E-cadherin not only for cell-cell contact but also for clonal propagation of hPSCs. Results indicate that use of continuous time-lapse imaging can distinguish cellular heterogeneity with respect to pluripotency as well as a subset of karyotypic abnormalities whose dynamic properties were monitored.