RIBOSOME SYNTHESIS DURING THE GROWTH-CYCLE OF SACCHAROMYCES-CEREVISIAE
RIBOSOME SYNTHESIS DURING THE GROWTH-CYCLE OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1002/yea.320100203
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发表时间：
1994-02-01
期刊：
影响因子：
2.6
通讯作者：
WARNER, JR
中科院分区：
文献类型：
--
作者：
JU, QD;WARNER, JR
我们已经测量了核糖体的含量，核糖体RNA的合成速率，以及核糖体蛋白的mRNA水平作为酿酒酵母的培养物通过生长周期。核糖体RNA和核糖体蛋白基因的转录在生长周期的早期阶段意外地消失，伴随着培养物的总RNA含量下降近50%，每个细胞的核糖体数量下降到小于最大值的25%。在此期间，细胞继续生长超过两个周期，最初以正常的对数生长速率生长，然后逐渐下降数小时。这些数据表明，细胞可以感觉到培养基内的不利变化，并通过调节其核糖体的合成和降解来做出反应。我们的结论是，细胞调节核糖体的合成和内容，根据其估计的增长潜力。
We have measured the content of ribosomes, the rate of synthesis of ribosomal RNA, and the level of the mRNA for ribosomal proteins as a culture of Saccharomyces cerevisiae passes through the growth cycle. The transcription of both ribosomal RNA and ribosomal protein genes disappears at an unexpectedly early stage in the growth cycle, accompanied by a decline in the total RNA content of the culture by nearly 50% and a decline in the number of ribosomes per cell to less than 25% of the maximum value. During this time the cells continue to grow through more than two doublings, initially at the normal log growth rate, which then decline gradually for several hours. The data suggest that the cell can sense an unfavorable change within the medium and responds by employing regulation of both synthesis and degradation of its ribosomes. We conclude that the cell regulates ribosome synthesis and content according to its estimate of the potential for growth.