A Perfusion Bioreactor for Engineering Bone Constructs: An In Vitro and In Vivo Study
A Perfusion Bioreactor for Engineering Bone Constructs: An In Vitro and In Vivo Study
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DOI：
10.1089/ten.tec.2010.0468
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发表时间：
2011-05-01
影响因子：
3
通讯作者：
Petite, Herve
中科院分区：
文献类型：
--
作者：
David, Bertrand;Bonnefont-Rousselot, Dominique;Petite, Herve
基于流化床概念设计的灌注生物反应器经验证可用于培养临床相关尺寸的骨结构。在这项研究中，天然珊瑚被用作三维支架。该生物材料是一种微孔、生物相容性、骨传导性和可吸收的支架。该灌注生物反应器在渗透压、pH值以及最重要的氧化应激方面提供了稳定的环境。在该系统中工程化的骨构建体导致比在静态条件下培养的那些显著更高的细胞增殖和均匀的细胞分布。特别是与临床环境中生物工程骨的生产相关的是，可以使用这种灌注生物反应器生产定制的骨构建体（每个骨构建体的体积高达30 cm 3）。最后，但并非最不重要的是，临床相关体积的骨结构，从而产生的皮下移植在绵羊时，显示成骨。
A perfusion bioreactor, which was designed based on fluidized bed concepts, was validated for the culture of bone constructs of clinically relevant size. For this study, natural coral has been used as three-dimensional scaffolds. This biomaterial is a microporous, biocompatible, osteoconductive, and absorbable scaffold. This perfusion bioreactor provided a stable environment in terms of osmolarity, pH, and, most importantly, oxidative stress. Bone constructs engineered in this system resulted in significantly higher cell proliferation and homogenous cell distribution than those cultured under static conditions. Particularly relevant to the production of bioengineered bone in a clinical setting, custom-made bone constructs (each one with volume up to 30 cm 3) could be produced using a such perfusion bioreactor. Last, but not least, the bone constructs of clinically relevant volume thus produced were shown to be osteogenic when transplanted subcutaneously in sheep.