Molded rigid polymer monoliths as separation media for capillary electrochromatography. 2. Effect of chromatographic conditions on the separation.
Molded rigid polymer monoliths as separation media for capillary electrochromatography. 2. Effect of chromatographic conditions on the separation.
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DOI：
10.1021/ac9713520
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发表时间：
1998-05
影响因子：
7.4
通讯作者：
E. C. Peters;M. Petro;F. Švec;J. Fréchet
中科院分区：
文献类型：
--
作者：
E. C. Peters;M. Petro;F. Švec;J. Fréchet
研究了毛细管电色谱条件对聚（甲基丙烯酸丁酯-co-二甲基丙烯酸乙烯酯-co-2-丙烯酰胺基-2-甲基-1-丙磺酸）整体柱性能的影响。发现流速与电场的强度以及移动的相的pH和组成成正比。在最佳流速为1.5 cm/min时，所有组成和孔隙率相同的整体毛细管柱的柱效均达到120，000板/m，而与其长度无关，其长度从30 cm变化到120 cm。聚合物分离介质表现出与反相色谱法中典型ODS填料相似的保留和选择性特性。除了“经典”使用的整体毛细管柱的电色谱分离的小分子在反相模式，较大的苯乙烯低聚物也分离等度洗脱条件下。此外，首次证明了分子量高达10（6）的聚苯乙烯标准品的电驱动尺寸排阻色谱。
The effect of chromatographic conditions on the performance of monolithic poly(butyl methacrylate-co-ethylene dimethacrylate-co-2-acrylamido-2-methyl-1-propanesulfonic acid) columns in capillary electrochromatography has been studied. The flow velocity was found to be proportional to the strength of the electric field and both the pH and the composition of the mobile phase. A column efficiency of 120,000 plates/m at the optimum flow velocity of 1.5 cm/min is achieved for all the monolithic capillary columns of identical composition and porosity, regardless of their length, which varied from 30 to 120 cm. The polymeric separation medium exhibits retention and selectivity properties similar to those of typical ODS packings for reversed-phase chromatography. In addition to the "classical" use of monolithic capillary columns for the electrochromatographic separation of small molecules in reversed-phase mode, larger styrene oligomers were also separated under isocratic elution conditions. In addition, the electroosmotically driven size exclusion chromatography of polystyrene standards with molecular weights up to 10(6) has been demonstrated for the first time.