LANGERHANS CELLS AS STIMULATOR CELLS IN THE MURINE PRIMARY EPIDERMAL-CELL LYMPHOCYTE-REACTION - ALTERATION BY UV-B IRRADIATION
LANGERHANS CELLS AS STIMULATOR CELLS IN THE MURINE PRIMARY EPIDERMAL-CELL LYMPHOCYTE-REACTION - ALTERATION BY UV-B IRRADIATION
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DOI：
10.1111/1523-1747.ep12500040
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发表时间：
1982-01-01
影响因子：
6.5
通讯作者：
WOLFF, K
中科院分区：
文献类型：
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作者：
ABERER, W;STINGL, G;WOLFF, K
免疫活性细胞的区域依赖功能，如：，诱导抗原特异性，同种异体和同基因T细胞活化，是高度紫外线敏感。由于表皮是唯一自然暴露于紫外线照射下的组织，我们进行了实验来研究小鼠表皮细胞（EC），特别是ia阳性朗格汉斯细胞（LC）是否能诱导同基因和异基因T细胞活化，如果是的话，这些功能是否会被紫外线照射改变。表皮细胞淋巴细胞培养（ELR）采用未辐照或辐照（1-40 mJ/cm2 UV-B） Balb/c (H-2d) EC作为刺激细胞，Balb/c或C57Bl/6 （H-2b）纯化的T淋巴细胞作为应答细胞。Balb/c EC不仅在C57Bl/6 T细胞中诱导了强烈的增殖反应（高峰反应：5-6天），而且在7-9天内也显著刺激了同基因T细胞。用特异性抗ia血清加补体预处理EC可消除其刺激能力，这表明LC在这些反应中至关重要。UV-B辐射导致EC的elr刺激能力呈剂量依赖性降低。UV-B剂量几乎消除了LC的ELR刺激能力，但对EC的活力没有影响，只是略微降低了EC的蛋白质合成能力。I区依赖的LC功能特别易受紫外线影响，这一发现可能为调节免疫反应的传入肢提供了有用的工具。
I region dependent functions of immunocompetent cells, i.,e., induction of antigen specific, allogeneic and syngeneic T cell activation, are highly UV susceptible. Since the epidermis is the only tissue which is naturally exposed to UV-irradiation, experiments were conducted to investigate whether murine epidermal cells (EC), particularly Ia-positive Langerhans cells (LC), could induce syngeneic and allogeneic T cell activation, and, if so, whether these functions could be altered by UV-irradiation. Epidermal cell-lymphocyte cultures (ELR) were established using either nonirradiated or irradiated (1-40 mJ/cm2 UV-B) Balb/c (H-2d) EC as stimulator cells and either Balb/c or C57Bl/6 (H-2b) purified T lymphocytes as responders. Balb/c EC not only induced a vigorous proliferative response in C57Bl/6 T cells (peak response: day 5-6) but also substantially stimulated syngeneic T cells (day 7-9). Pretreatment of EC with specific anti-Ia serum plus complement abolished their stimulatory capacities which demonstrates that LC are of critical importance in these reactions. UV-B irradiation of EC resulted in a dose-dependent reduction of their ELR-stimulatory capacities. UV-B doses which virtually abolished the ELR stimulatory capacity of LC did not affect EC viability and only marginally reduced the protein synthesizing capacity of EC. The finding that I region dependent LC functions are particularly UV-susceptible may provide a useful tool for the modulation of the afferent limb of the immune response.