Neurochemical fine tuning of a peripheral tissue: peptidergic and aminergic regulation of fluid secretion by Malpighian tubules in the tobacco hawkmoth M. sexta.
Neurochemical fine tuning of a peripheral tissue: peptidergic and aminergic regulation of fluid secretion by Malpighian tubules in the tobacco hawkmoth M. sexta.
复制标题
DOI：
--
复制
发表时间：
2002-07
期刊：
影响因子：
--
通讯作者：
N. Skaer;D. Nässel;S. Maddrell;N. Tublitz
中科院分区：
文献类型：
--
作者：
N. Skaer;D. Nässel;S. Maddrell;N. Tublitz
本文研究了不同肽类化合物对烟草天蛾马氏管分泌液体的影响。使用新开发的pharate成人马氏管生物测定，我们表明，3速激肽相关肽（TRP），白细胞激肽I，5-羟色胺（5-HT），章鱼胺，心脏加速肽1a，1b和2c，环磷酸鸟苷和环磷酸腺苷每一个引起pharate成人小管的液体分泌率增加。尽管生物胺和某些肽的可能激素来源是已知的，但TRP的分布以前在M.六。因此，我们使用抗TRP抗血清进行免疫细胞化学。我们发现在M. sexta中枢神经系统（CNS）。幼虫的大脑含有大约60个TRP免疫阳性细胞，而在包括视叶在内的成体大脑中约有100个这样的细胞。腹神经索的每个神经节也含有TRP样免疫反应细胞。在中枢神经系统中没有发现含TRP的神经分泌细胞，但中肠的内分泌细胞与抗血清发生反应。我们提出的假设，在昆虫的生理系统的激素控制可能并不总是涉及组织特异性激素，迫使刻板的反应，在其目标系统。相反，在细胞外液中可能存在以化学语言形式的连续信息广播，身体的某些或所有部分随时都在不断地对这种信息广播作出反应，这确保了比其他方式更有效和高效的功能协调。
The actions of various peptides and other compounds on fluid secretion by Malpighian tubules in the tobacco hawkmoth Manduca sexta sexta are investigated in this study. Using a newly developed pharate adult Malpighian tubule bioassay, we show that three tachykinin-related peptides (TRPs), leucokinin I, serotonin (5-HT), octopamine, the cardioacceleratory peptides 1a, 1b and 2c, cGMP and cAMP each cause an increase in the rate of fluid secretion in pharate adult tubules. Whereas the possible hormonal sources of biogenic amines and some of the peptides are known, the distribution of TRPs has not been investigated previously in M. sexta. Thus we performed immunocytochemistry using an anti-TRP antiserum. We show the presence of TRP-like material in a small subset of cells in the M. sexta central nervous system (CNS). The larval brain contains approximately 60 TRP-immunopositive cells and there are approximately 100 such cells in the adult brain including the optic lobes. Every ganglion of the ventral nerve cord also contains TRP-like immunoreactive cells. No TRP-containing neurosecretory cells were seen in the CNS, but endocrine cells of the midgut reacted with the antiserum. We propose the hypothesis that the control in insects of physiological systems by hormones may not always involve tissue-specific hormones that force stereotypical responses in their target systems. Instead, there may exist in the extracellular fluid a continuous broadcast of information in the form of a chemical language to which some or all parts of the body continuously respond on a moment-to-moment basis, and which ensures a more effective and efficient coordination of function than could be achieved otherwise.