Peritoneal macrophage from male and female SJL mice differ in IL-10 expression and macrophage maturation
Peritoneal macrophage from male and female SJL mice differ in IL-10 expression and macrophage maturation
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DOI：
10.1189/jlb.0711351
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发表时间：
2012-04-01
影响因子：
5.5
通讯作者：
Stohlman, Stephen A.
中科院分区：
文献类型：
--
作者：
Hussain, Shabbir;Stohlman, Stephen A.
将蛋白质和颗粒抗原注射到雄性SJL小鼠的腹腔中优先激活分泌Th 2细胞因子的T细胞。相同的女性免疫激活分泌Th 1细胞因子的T细胞。在幼稚雄性和雌性之间比较CD 11b（+）F4/80（hi）LPM和CD 11b（+）F4/80（io）SPM群体，以确定它们在支持Th 1与Th 2 T细胞活化差异中的作用。MHC II类分子、共刺激分子和MR的表达无性别依赖性差异。免疫诱导两种性别中CD 11b（io）F4/80（io）细胞的流入。CD 11b（io）F4/80（io）细胞主要由Ly 6C（hi）单核细胞组成，其成熟为Ly 6C（-）SPM亚群。免疫后，相等频率的LPM已经吸收抗原。然而，与女性相比，男性中已摄取抗原的CD 11b（io）F4/80（io）群体显著减少。与初始巨噬细胞相似，免疫雄性和雌性中的抗原阳性细胞未表现出表型差异。然而，与雌性动物相比，雄性动物中的Ly 6C（-）F4/80（+）细胞较少，与抗原阳性细胞数量减少一致。此外，与雌性来源的抗原阳性CD 11b（io）F4/80（io）细胞中主要的IL-12 mRNA表达相比，雄性中摄取抗原的CD 11b（io）F4/80（io）细胞表达增加的IL-10和有限的IL-12 mRNA。IL-10阻断使雄性中Ly 6C（-）F4/80（+）细胞的频率增加至雌性中的频率，表明雄性SJL小鼠中Th 2 T细胞的优先活化与IL-10表达增加和有限的抗原呈递相关，这是由于在IL-10的影响下巨噬细胞成熟减少所致。J. Leukoc. 91：571-579; 2012.
Injection of proteins and particulate antigens into the peritoneal cavity of male SJL mice preferentially activates T cells secreting Th2 cytokines. Identical immunizations of females activate T cells secreting Th1 cytokines. CD11b(+)F4/80(hi) LPM and CD11b(+)F4/80(io) SPM populations were compared between naive males and females to define their role in supporting differential Th1 versus Th2 T cell activation. No sex-dependent differences in the expression of MHC class II, costimulatory molecules, and MR were detected. Immunization induced influx of CD11b(io)F4/80(io) cells in both sexes. CD11b(io)F4/80(io) cells consist predominantly of Ly6C(hi) monocytes, which mature into a Ly6C(-) SPM subset. Following immunization, equivalent frequencies of LPM had taken up antigen. However, the CD11b(io)F4/80(io) population, which had taken up antigen, was decreased significantly in males compared with females. Similar to naive macrophages, antigen-positive cells in immunized males and females exhibited no phenotypic differences. However, fewer Ly6C(-)F4/80(+) cells were present in males compared with females, consistent with the reduced number of antigen-positive cells. Furthermore, CD11b(io)F4/80(io) cells, which had taken up antigen in males, expressed increased IL-10 and limited IL-12 mRNA compared with the predominant IL-12 mRNA expression in female-derived, antigen-positive CD11b(io)F4/80(io) cells. IL-10 blockade increased the frequency of Ly6C(-)F4/80(+) cells in males to the frequency in females, suggesting that preferential activation of Th2 T cells in male SJL mice is associated with increased IL-10 expression and limited antigen presentation as a result of decreased macrophage maturation under the influence of IL-10. J. Leukoc. Biol. 91: 571-579; 2012.