MECHANISMS OF COBALT(II) UPTAKE INTO V79-CHINESE HAMSTER-CELLS
MECHANISMS OF COBALT(II) UPTAKE INTO V79-CHINESE HAMSTER-CELLS
复制标题
DOI：
10.1007/bf01973391
复制
发表时间：
1992-10-01
影响因子：
6.1
通讯作者：
BEYERSMANN, D
中科院分区：
文献类型：
--
作者：
KASTEN, U;HARTWIG, A;BEYERSMANN, D
V79中国仓鼠细胞被用作模型的Co（II）摄取到哺乳动物细胞的特性，以及所涉及的机制。Co（II）以剂量和时间依赖性方式被摄取。在高达400 μ M CoCl 2的测试浓度范围内，吸收呈指数型而不饱和。此外，在升高的细胞外Co（II）剂量下（在200 μ M时高达16倍），存在高的细胞内钴积累。Co（II）吸收的时间过程在约8-12小时后显示出最大值，在最长测试孵育时间（24小时）后没有进一步的变化。Co（II）进入V79细胞的摄取似乎由多种机制介导：由于哇巴因显著降低了Co（II）的摄取，因此，活性的、能量消耗的转运，如离子泵和内吞作用（Na+/K+ ATP酶抑制剂），N-乙基马来酰亚胺（Ca 2 +/Mg 2 + ATP酶和Na+/K+ ATP酶的抑制剂），氯丙嗪（钙调蛋白拮抗剂和Ca 2 +/Mg 2 + ATP酶抑制剂）以及内吞作用抑制剂氯喹。此外，碘乙酸和氰化钾这两种产生ATP耗竭的试剂导致细胞内钴浓度降低。由于4，4 '-二异硫氰基芪-2，2-二磺酸不具有抑制作用，因此可以排除通过阴离子通道的摄取
V79 Chinese hamster cells were used as a model for the characterization of the Co(II) uptake into mammalian cells as well as the mechanisms involved. Co(II) was taken up in a dose and time dependent manner. The uptake was exponential without saturation in the tested concentration range up to 400 muM CoCl2. Furthermore, there was a high intracellular cobalt accumulation at elevated extracellular Co(II) doses (up to 16 fold at 200 muM). The time course of Co(II) uptake showed a maximum after about 8-12 h with no further change after the longest tested incubation time (24 h). The uptake of Co(II) into V79 cells seems to be mediated by multiple mechanisms: active, energy consuming transport like ion pumps and endocytosis, since the Co(II) uptake was significantly reduced by ouabain (an inhibitor of the Na+/K+ATPase), N-ethylmaleinimide (an inhibitor of the Ca2+/Mg2+ATPase and the Na+/K+ATPase), chlorpromazine (a calmodulin antagonist and inhibitor of the Ca2+/Mg2+ ATPase) as well as by the endocytosis inhibitor chloroquine. Furthermore, the two agents iodoacetate and potassium cyanide, which produce ATP depletion, resulted in a diminution of the intracellular cobalt concentration. An uptake through anion channels could be excluded, since 4,4'-diisothiocyanostilbene-2,2-disulphonic acid was not inhibitory