MORPHOLOGICAL AND FUNCTIONAL CONSEQUENCES OF PRENATAL EXPOSURE TO DIETHYLSTILBESTROL IN RAT
MORPHOLOGICAL AND FUNCTIONAL CONSEQUENCES OF PRENATAL EXPOSURE TO DIETHYLSTILBESTROL IN RAT
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DOI：
10.1095/biolreprod19.4.854
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发表时间：
1978-01-01
影响因子：
3.6
通讯作者：
BOYLAN, ES
中科院分区：
文献类型：
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作者：
BOYLAN, ES
在妊娠第2或第3个月用己烯雌酚（DES）治疗妊娠大鼠，以确定子宫内暴露于DES对子代的影响。DES剂量超过120亩。G阻止分娩，导致男性和女性胎儿在足月时乳头突出。对大多数孕鼠不影响妊娠和分娩的DES最高剂量为120 .mu。妊娠晚期G， 1.2 .mu。G在妊娠中期。在妊娠晚期暴露于DES的雌性后代表现出乳头和阴道开口的早熟发育。显微镜下，高剂量时乳头形态异常。成熟时，大量动物暴露在120亩土地上。外生殖器和生殖器官，尤其是卵巢有异常。这些乳头和生殖道的结构异常很可能是这些雌性与未经治疗的雄性交配时生育能力受损的原因，也是它们支持幼崽断奶的能力下降的原因。而暴露于1.2 μ m的部分动物外生殖器出现异常。g妊娠晚期DES，这些动物的生育能力和生殖器官形态正常。暴露在1.2亩。妊娠中期DES与雌性后代在新生儿或成年时的形态异常或这些动物的生育能力受损无关。所有实验组的男性同胞在结构或功能上都没有受到影响。这些数据表明，大鼠是研究雌激素对多种胎儿组织影响的有用模型系统。
Pregnant rats were treated with diethylstilbestrol (DES) during the 2nd or 3rd trimester of pregnancy to determine the effects of exposure to DES in utero on the offspring. Doses of DES in excess of 120 .mu.g prevented parturition and resulted in the development of prominent nipples in both the male and female fetuses at term. The highest dose of DES which did not interfere with pregnancy and parturition in a majority of the pregnant animals was 120 .mu.g in the 3rd trimester and 1.2 .mu.g in the 2nd trimester. Female offspring exposed to DES during the 3rd trimester displayed precocious development of the nipples and opening of the vagina. Microscopically, nipple morphology was abnormal at the higher doses. At maturity, substantial numbers of animals exposed to 120 .mu.g DES had abnormalities of the external genitalia and reproductive organs, especially the ovaries. These structural abnormalities of the nipples and reproductive tracts are likely to account for the impaired fertility seen when these females were mated to untreated males and for their reduced capacity to support pups to weaning. While abnormalities of the external genitalia were noted in some animals exposed to 1.2 .mu.g DES in the 3rd trimester, these animals were normal with respect to fertility and morphology of the reproductive organs. Exposure to 1.2 .mu.g DES during the 2nd trimester was not associated with morphological abnormalities in the female offspring as neonates or adults or impairment of the fertility of these animals. Male sibs from all experimental groups did not appear structurally or functionally affected. These data demonstrate that the rat is a useful model system to study the effects of estrogenic hormones on a variety of fetal tissues.