LEVELS OF HEPATOCYTE GROWTH-FACTOR IN MATERNAL SERUM AND AMNIOTIC-FLUID
LEVELS OF HEPATOCYTE GROWTH-FACTOR IN MATERNAL SERUM AND AMNIOTIC-FLUID
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DOI：
10.1016/0002-9378(95)90370-4
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发表时间：
1995-09-01
影响因子：
9.8
通讯作者：
TOMODA, Y
中科院分区：
文献类型：
--
作者：
HORIBE, N;OKAMOTO, T;TOMODA, Y
目的：探讨妊娠期母血和羊水中肝细胞生长因子的水平。我们还证明了生产和分泌的肝细胞生长因子胎盘和羊膜在不同阶段的gestation.Study设计：肝细胞生长因子水平在母体血清（n = 219），脐带血（n = 20），羊水样本（n = 90）进行了测定酶联免疫吸附试验。胎盘和羊膜分泌的肝细胞生长因子通过测量释放到培养上清液中的量来评估。结果：妊娠10周前，大多数孕妇血清中的肝细胞生长因子水平低于检测限。此后水平显著增加，并继续增加，直到任期。另一方面，妊娠20至29周之间羊水中的浓度明显高于30周后。从胎盘组织单位重量的肝细胞生长因子分泌似乎在整个怀孕期间没有变化。它的分泌从羊膜，但是，约300至400倍高，在孕中期相比，在term. CONCLUSION：胎盘和羊膜产生和分泌肝细胞生长因子，这表明其在胎儿生长和胎盘的生长和分化的作用。
OBJECTIVE: The purpose of our study was to investigate hepatocyte growth factor levels in maternal serum and amniotic fluid during pregnancy. We also demonstrated production and secretion of hepatocyte growth factor by placenta and amnion at different stages of gestation.STUDY DESIGN: Hepatocyte growth factor levels in maternal serum (n = 219), cord blood (n = 20), and amniotic fluid samples (n = 90) were measured by an enzyme-linked immunosorbent assay. The secretion of hepatocyte growth factor by placenta and amnion was evaluated by measuring the amount released into the culture supernatant.RESULTS: Most hepatocyte growth factor levels in maternal serum were below the detection limit before 10 weeks of pregnancy. Levels increased significantly thereafter and continued to increase until term. On the other hand, levels in amniotic fluid were significantly higher between 20 and 29 weeks of gestation than after 30 weeks. Hepatocyte growth factor secretion from the placental tissue per weight seemed unchanged throughout pregnancy. Its secretion from amnion was, however, approximately 300 to 400-fold higher in the second trimester compared with that at term.CONCLUSION: Both placenta and amnion produce and secrete hepatocyte growth factor, suggesting its role in fetal growth and the growth and differentiation of placenta.