Evaluation of protective effects of fish oil against oxidative damage in rats exposed to methylmercury
Evaluation of protective effects of fish oil against oxidative damage in rats exposed to methylmercury
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DOI：
10.1016/j.ecoenv.2010.10.012
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发表时间：
2011-03-01
影响因子：
6.8
通讯作者：
Barbosa, Fernando, Jr.
中科院分区：
文献类型：
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作者：
Grotto, Denise;Vicentini, Juliana;Barbosa, Fernando, Jr.
本研究评估了鱼油对亚慢性暴露大鼠中甲基汞 (MeHg) 促进的氧化损伤的可能保护作用。与对照组相比，接触甲基汞的动物中观察到谷胱甘肽过氧化物酶和过氧化氢酶活性降低，谷胱甘肽水平降低。甲基汞暴露也与 DNA 损伤有关。给暴露于甲基汞的动物服用鱼油并没有改善酶活性或谷胱甘肽水平。另一方面，鱼油处理观察到显着的 DNA 保护作用（约 30%）。施用甲基汞后，无论是否同时施用鱼油，大鼠肝脏、肾脏、心脏或大脑中的总汞浓度没有差异。组织病理学分析显示，接触甲基汞后，大鼠组织中出现显着的白细胞浸润，但在同时施用鱼油后，这种效应显着降低。总而言之，我们的研究结果表明，即使在低水平甲基汞接触后，也会出现氧化损伤，并且鱼油具有保护作用。这种保护似乎与大鼠组织中的抗氧化防御或汞重新分布无关。这可能是由于鱼油的抗炎作用。 (C) 2010 Elsevier Inc. 保留所有权利。
The present study evaluates a possible protective effect of fish oil against oxidative damage promoted by methylmercury (MeHg) in sub-chronically exposed rats. Reduced glutathione peroxidase and catalase enzyme activity and reduced glutathione levels were observed in MeHg-exposed animals compared to controls. Methylmercury exposure was also associated with DNA damage. Administration of fish oil to the methylmercury-exposed animals did not ameliorate enzyme activity or glutathione levels. On the other hand, a significant DNA protective effect (about 30%) was observed with fish oil treatment. There were no differences in the total mercury concentration in rat liver, kidney, heart or brain after MeHg administration with or without fish oil co-administration. Histopathological analyses showed a significant leukocyte infiltration in rat tissues after MeHg exposure, but this effect was significantly reduced after co-administration of fish oil.Taken together, our findings demonstrate oxidative damage even after low-level MeHg exposure and the protective effect of fish oil. This protection seems not to be related to antioxidant defenses or mercury re-distribution in rat tissues. It is probably due to the anti-inflammatory effects of fish oil. (C) 2010 Elsevier Inc. All rights reserved.