The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome
The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome
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DOI：
10.1099/vir.0.18606-0
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发表时间：
2003-01-01
影响因子：
3.8
通讯作者：
Hayward, GS
中科院分区：
文献类型：
--
作者：
Davison, AJ;Dolan, A;Hayward, GS
由于人巨细胞病毒（HCMV）基因组的大小和复杂性，以及实验室毒株在适应培养生长过程中发生了缺失和重排，野生型HCMV的基因组成尚未完全了解。我们已经测定了黑猩猩巨细胞病毒（CCMV）的序列（241087bp），并将其与已发表的来自实验室毒株AD169和托莱多的HCMV序列进行了比较，目的是阐明野生型HCMV的基因含量。HCMV和CCMV基因组有一定程度的差异，但基本呈线性排列。基于潜在蛋白质编码区域和其他序列特征的保守性，我们排除了先前提出的51个HCMV开放阅读框（ORF），对24个进行了修改（包括多个外显子的归属），并提出了10个新基因。在已发表的HCMV序列中发现了几个错误。我们目前在CCMV中识别出165个基因，在AD169中识别出145个基因；相比之下，1990年对AD169估计有189个独特基因。我们对野生型HCMV基因组成的最佳估计是164到167个基因。
The gene complement of wild-type human cytomegalovirus (HCMV) is incompletely understood, on account of the size and complexity of the viral genome and because laboratory strains have undergone deletions and rearrangements during adaptation to growth in culture. We have determined the sequence (241 087 bp) of chimpanzee cytomegalovirus (CCMV) and have compared it with published HCMV sequences from the laboratory strains AD169 and Toledo, with the aim of clarifying the gene content of wild-type HCMV. The HCMV and CCMV genomes are moderately diverged and essentially collinear. On the basis of conservation of potential protein-coding regions and other sequence features, we have discounted 51 previously proposed HCMV ORFs, modified the interpretations for 24 (including assignments of multiple exons) and proposed ten novel genes. Several errors were detected in the published HCMV sequences. We presently recognize 165 genes in CCMV and 145 in AD169; this compares with an estimate of 189 unique genes for AD169 made in 1990. Our best estimate for the complement of wild-type HCMV is 164 to 167 genes.