Lipidomics reveals the dynamics of lipid profile altered by omega‐3 polyunsaturated fatty acid supplementation in healthy people
Lipidomics reveals the dynamics of lipid profile altered by omega‐3 polyunsaturated fatty acid supplementation in healthy people
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DOI：
10.1111/1440-1681.13285
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发表时间：
2020-02
影响因子：
2.9
通讯作者：
Meng Yan;Wenbin Cai;Tong Hua;Qian Cheng;Ding Ai;Hong-feng Jiang;Xu Zhang
中科院分区：
文献类型：
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作者：
Meng Yan;Wenbin Cai;Tong Hua;Qian Cheng;Ding Ai;Hong-feng Jiang;Xu Zhang
甘油磷脂（GP）和鞘脂（SP）是人体内重要的脂质成分，发挥着重要的生物学功能。Omega-3多不饱和脂肪酸（n-3 PUFA）是重要的营养素，其补充剂通常用于预防某些疾病。然而，n-3 PUFA对人甘油磷脂组和鞘脂组的影响尚不清楚。我们使用靶向脂质组学来研究健康个体在补充n-3 PUFA 3、7、14和21天后的GP和SP谱。使用模糊c-均值聚类将脂质种类聚类为六类，反映n-3 PUFA补充后不同的含量变化模式。在具有显著变化的含量的物种中，溶血磷脂是最敏感的;它们的含量在第3天开始增加。磷脂酰丝氨酸含量在后期增加。大多数磷脂酰胆碱和烷基磷脂酰胆碱的含量在第21天下降。脂质种类的相关网络分析表明，参与溶血磷脂和磷脂酰丝氨酸代谢的一些酶受n-3 PUFA的调节。在第21天时，肌酸激酶MB（CK-MB）、尿素、葡萄糖、甘油三酯和总胆红素的水平被n-3 PUFA改变。相关性分析显示，CK-MB水平与溶血磷脂酸、溶血磷脂酰胆碱、溶血磷脂酰乙醇胺和磷脂酰丝氨酸类中的物种水平呈负相关，这些物种通过补充n-3 PUFA而增加。通过本工作的分析，我们揭示了n-3 PUFAs对GP和SP代谢的规律，为n-3 PUFAs的临床应用提供了药理学依据。
Glycerophospholipids (GPs) and sphingolipids (SPs) are important lipid components in the body and play biological functions. Omega‐3 polyunsaturated fatty acids (n‐3 PUFAs) are important nutrients, and their supplements are commonly used for preventing some diseases. However, the effect of n‐3 PUFAs on the human glycerophospholipidome and sphingolipidome is unclear. We used targeted lipidomics to study the GP and SP profile of healthy individuals after supplementation with n‐3 PUFAs for 3, 7, 14 and 21 days. Fuzzy c‐means clustering was used to cluster the lipid species into six classes reflecting different changed‐content patterns after n‐3 PUFA supplementation. Among the species with significantly changed content, lysophospholipids were the most sensitive; their content started to increase on day 3. The content of phosphatidylserines increased at a later stage. The content of most of the phosphatidylcholines and alkylphosphatidylcholines decreased on day 21. A correlation network analysis of lipid species suggested that some enzymes involved in the metabolism of lysophospholipids and phosphatidylserines were regulated by n‐3 PUFAs. Levels of creatine kinase‐MB (CK‐MB), urea, glucose, triglycerides and total bilirubin were altered by n‐3 PUFA at 21 days. Correlation analysis revealed that the level of CK‐MB was negatively correlated with those of species in lysophosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine and phosphatidylserine classes, which were increased by n‐3 PUFA supplementation. With the analysis in this work, we demonstrated the regular pattern of n‐3 PUFAs on GP and SP metabolism, which provides a pharmacological basis for n‐3 PUFAs for clinical application.