Impact of Growth Phase, Pigment Adaptation, and Climate Change Conditions on the Cellular Pigment and Carbon Content of Fifty‐One Phytoplankton Isolates
Impact of Growth Phase, Pigment Adaptation, and Climate Change Conditions on the Cellular Pigment and Carbon Content of Fifty‐One Phytoplankton Isolates
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DOI：
10.1111/jpy.13279
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发表时间：
2022-07
影响因子：
2.9
通讯作者：
A. Neeley;M. Lomas;A. Mannino;Crystal S. Thomas;R. Vandermeulen
中科院分区：
文献类型：
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作者：
A. Neeley;M. Lomas;A. Mannino;Crystal S. Thomas;R. Vandermeulen
由于浮游植物在水生生态系统中的重要性，过去十年来，对估算全球海洋浮游植物生物量和群落组成的模型的需求有所增加。此外，气候变化的影响，包括二氧化碳（CO2）升高，分层增加和海洋表面温度升高，可能会影响全球海洋中浮游植物群落的组成。化学分类学的方法是有用的建模浮游植物群落组成的标记色素归一化叶绿素a（Chla）。然而，光合色素，特别是叶绿素a，是敏感的营养和光照条件。细胞碳不太敏感，因此使用碳生物质代替可能提供一种替代方法。为此，在51株全球相关的，培养的浮游植物细胞色素和碳浓度进行了测量。计算每种菌株的色素与叶绿素a和色素与碳的比值。对于25个菌株，在两个生长阶段进行测量。虽然观察到生长阶段之间存在一些差异，但未超过类内差异。测量了多个前沟藻（Amphidinium carterae）、白氏双沟藻（Ditylum brightwellii）和赤潮异弯藻（Heterosigma akashiwo）菌株，以确定培养时间是否影响色素和碳组成。未观察到细胞色素或碳含量的明显趋势。最后，气候变化条件对色素比例的潜在影响进行了评估，使用多应激实验，包括增加平均光照，温度和升高的pCO2对三个物种：海链藻海洋，Ostreococcus lucimarinus，聚球藻。最大的差异在色素与碳的比例中观察到，而标记色素在很大程度上与Chl a共变。这些意见的化学分类学应用的影响进行了讨论。
Owing to their importance in aquatic ecosystems, the demand for models that estimate phytoplankton biomass and community composition in the global ocean has increased over the last decade. Moreover, the impacts of climate change, including elevated carbon dioxide (CO2), increased stratification, and warmer sea surface temperatures, will likely shape phytoplankton community composition in the global ocean. Chemotaxonomic methods are useful for modeling phytoplankton community composition from marker pigments normalized to chlorophyll a (Chl a). However, photosynthetic pigments, particularly Chl a, are sensitive to nutrient and light conditions. Cellular carbon is less sensitive, so using carbon biomass instead may provide an alternative approach. To this end, cellular pigment and carbon concentrations were measured in 51 strains of globally relevant, cultured phytoplankton. Pigment‐to‐Chl a and pigment‐to‐carbon ratios were computed for each strain. For 25 strains, measurements were taken during two growth phases. While some differences between growth phases were observed, they did not exceed within‐class differences. Multiple strains of Amphidinium carterae, Ditylum brightwellii and Heterosigma akashiwo were measured to determine whether time in culture influenced pigment and carbon composition. No appreciable trends in cellular pigment or carbon content were observed. Lastly, the potential impact of climate change conditions on the pigment ratios was assessed using a multistressor experiment that included increased mean light, temperature, and elevated pCO2 on three species: Thalassiosira oceanica, Ostreococcus lucimarinus, and Synechococcus. The largest differences were observed in the pigment‐to‐carbon ratios, while the marker pigments largely covaried with Chl a. The implications of these observations to chemotaxonomic applications are discussed.