Identification of key virus?prokaryote infection pairs that contribute to viral shunt in a freshwater lake
Identification of key virus?prokaryote infection pairs that contribute to viral shunt in a freshwater lake
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鉴定导致淡水湖中病毒分流的关键病毒？原核生物感染对
DOI：
10.1101/2023.02.05.527221
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Shimizu Yoshihisa
中科院分区：
文献类型：
--
作者：
Shen Shang;Tominaga Kento;Tsuchiya Kenji;Matsuda Tomonari;Yoshida Takashi;Shimizu Yoshihisa
病毒以密度或频率依赖的方式感染和杀死多产的原核生物，并影响碳循环。然而，层化转变，包括层化和去层化阶段，对原核生物/病毒群落的变化以及它们之间的相互作用的影响尚不清楚。我们对日本琵琶湖一个大而深的淡水湖进行了为期一年的月度表层和深层调查，并对其原核生物产量和原核生物/病毒元基因组进行了分析。我们的分析(包括1608种原核生物和13761种病毒)表明，当原核生物产量较高时，19种原核生物可能受到病毒的抑制，占∼总丰度的40%。这表明，一小部分原核生物贡献了大量的原核生物丰度，这些原核生物被病毒感染和裂解，推动了淡水湖中的病毒分流。此外，我们还发现，年度垂直混合可能会在表层和深层之间产生类似的群落变化速率。这一发现可能对理解未来淡水湖分层受到全球变暖影响时群落如何变化有价值。
Viruses infect and kill productive prokaryotes in a density-or frequency-dependent manner and affect carbon cycling. However, the effects of the stratification transition, including the stratified and destratified periods, on the changes in prokaryotic/viral communities and the interactions among them remain unclear. We conducted a monthly survey of the surface and deep layers of a large and deep freshwater lake (Lake Biwa, Japan) for a year and analyzed the prokaryotic production and prokaryotic/viral metagenome. Our analysis (including 1 608 prokaryotes and 13 761 viruses) revealed that 19 prokaryotic species, accounting for ∼40% of total abundance, might be suppressed by viruses when prokaryotic production is higher. This suggests that a small proportion of prokaryotes contribute to a large amount of prokaryotic abundance, and these prokaryotes are infected and lysed by viruses, driving the viral shunt in the freshwater lake. Furthermore, we found that annual vertical mixing might yield a similar rate of community change between the surface and deep layers. This finding might be valuable in understanding how the communities change when the stratification of freshwater lakes is affected by global warming in the future.