Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings.
Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings.
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DOI：
10.1093/jxb/erp134
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发表时间：
2009
影响因子：
6.9
通讯作者：
Yanagawa Y
中科院分区：
文献类型：
--
作者：
Igawa T;Fujiwara M;Takahashi H;Sawasaki T;Endo Y;Seki M;Shinozaki K;Fukao Y;Yanagawa Y
真核细胞中的大多数蛋白质都是根据高度调控的机制进行修饰，以实现特定的功能，实现定位、稳定和运输。蛋白质泛素化是真核细胞中主要的翻译后修饰之一。为了获得与拟南芥中泛素(Ub)依赖的调控系统相关的蛋白质组数据集，在自然条件下用抗Ub抗体进行了亲和纯化。通过MS/MS分析，共鉴定出196个差异蛋白，分别代表251个差异基因。鉴定的蛋白质涉及代谢(23.0%)、应激反应(21.4%)、翻译(16.8%)、运输(6.7%)、细胞形态(3.6%)和信号转导(1.5%)，此外还涉及蛋白水解酶(16.8%)，其中包括蛋白酶体亚基(14.3%)。根据可能的泛素化靶向信号基序、凝胶内迁移率和以前的报道，78个被鉴定的蛋白质被归类为泛素化蛋白，其余的被推测为泛素化蛋白的相关蛋白。蛋白酶体抑制剂抑制了三种泛素化蛋白的降解，表明这些蛋白受Ub/蛋白酶体依赖的蛋白降解的调节。
The majority of proteins in eukaryotic cells are modified according to highly regulated mechanisms to fulfill specific functions and to achieve localization, stability, and transport. Protein ubiquitination is one of the major post-translational modifications occurring in eukaryotic cells. To obtain the proteomic dataset related to the ubiquitin (Ub)-dependent regulatory system in Arabidopsis, affinity purification with an anti-Ub antibody under native condition was performed. Using MS/MS analysis, 196 distinct proteins represented by 251 distinct genes were identified. The identified proteins were involved in metabolism (23.0%), stress response (21.4%), translation (16.8%), transport (6.7%), cell morphology (3.6%), and signal transduction (1.5%), in addition to proteolysis (16.8%) to which proteasome subunits (14.3%) is included. On the basis of potential ubiquitination-targeting signal motifs, in-gel mobilities, and previous reports, 78 of the identified proteins were classified as ubiquitinated proteins and the rest were speculated to be associated proteins of ubiquitinated proteins. The degradation of three proteins predicted to be ubiquitinated proteins was inhibited by a proteasome inhibitor, suggesting that the proteins were regulated by Ub/proteasome-dependent proteolysis.
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影响因子：
7.2
作者：
Hatfield, PM;Gosink, MM;Vierstra, RD
通讯作者：
Vierstra, RD
影响因子：
5.1
作者：
Manzano, Concepcion;Abraham, Zamira;Del Pozo, Juan C.
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Del Pozo, Juan C.
影响因子：
10.5
作者：
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Deng, XW
影响因子：
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作者：
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作者：
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