Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons.
Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons.
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二维和球状间充质干细胞以及间充质干细胞衍生的微泡和视网膜感光神经元的共培养物分泌的信号因子
DOI：
10.1155/2017/2730472
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发表时间：
2017
影响因子：
4.3
通讯作者：
Jiang B
中科院分区：
文献类型：
--
作者：
Xie L;Mao M;Zhou L;Zhang L;Jiang B
我们的目标是确定在2D单层（2D-MSCs）、球状（spheroid MSCs）和源自2D-MSCs或球状MSCs和视网膜感光神经元的微泡（MVs）共培养中培养的MSCs分泌的信号因子水平。我们以相同数量的2D-MSCs、球形MSCs和球形MSCs衍生的细胞进行播种。3种培养条件下分离的mv与661W细胞孵育。从这些培养条件的条件培养基中，51个信号因子的水平用基于珠的试验进行定量。我们发现IL-8、IL-6和GROα是前三位最丰富的信号因子。此外，与2d MSCs相比，球形MSCs在条件培养基中11种细胞因子和IL-2Rα水平显著升高。最后，为了测试这些因子的表达增强是否反映了免疫调节活性的改变，我们评估了2d - msc - mv和3d - msc - mv对CD14+细胞化学吸引的影响。与2d - msc - mv相比，3d - msc - mv显著降低了CD14+细胞的趋化指数。我们的结果表明，球形培养条件提高了MSCs选择性分泌信号因子的能力。此外，与2d - msc - mv相比，3d - msc - mv还具有增强的促进信号因子分泌的能力，可能具有增强的免疫调节活性，可能是视网膜退行性疾病更好的再生治疗方法。
We aim to identify levels of signal factors secreted by MSCs cultured in 2D monolayers (2D-MSCs), spheroids (spheroids MSCs), and cocultures of microvesicles (MVs) derived from 2D-MSCs or spheroid MSCs and retinal photoreceptor neurons. We seeded 2D-MSCs, spheroid MSCs, and cells derived from spheroids MSCs at equal numbers. MVs isolated from all 3 culture conditions were incubated with 661W cells. Levels of 51 signal factors in conditioned medium from those cultured conditions were quantified with bead-based assay. We found that IL-8, IL-6, and GROα were the top three most abundant signal factors. Moreover, compared to 2D-MSCs, levels of 11 cytokines and IL-2Rα were significantly increased in conditioned medium from spheroid MSCs. Finally, to test if enhanced expression of these factors reflects altered immunomodulating activities, we assessed the effect of 2D-MSC-MVs and 3D-MSC-MVs on CD14+ cell chemoattraction. Compared to 2D-MSC-MVs, 3D-MSC-MVs significantly decreased the chemotactic index of CD14+ cells. Our results suggest that spheroid culture conditions improve the ability of MSCs to selectively secrete signal factors. Moreover, 3D-MSC-MVs also possessed an enhanced capability to promote signal factors secretion compared to 2D-MSC-MVs and may possess enhanced immunomodulating activities and might be a better regenerative therapy for retinal degenerative diseases.