Construction of cDNA Clone and Nucleotde Sequence of RNA3 of Cucumber Mosaic Virus Isolate Xb from Banana
Construction of cDNA Clone and Nucleotde Sequence of RNA3 of Cucumber Mosaic Virus Isolate Xb from Banana
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作者：
Wang Qi-ying
利用两对引物对黄瓜花叶病毒香蕉亚群株系（CMV-Xb）的RNA 3基因进行RT-PCR扩增，并对其进行核苷酸和蛋白质水平的分析。结果表明，CMV-Xb基因由2205个核苷酸组成，有两个开放阅读框，5 '端的一个开放阅读框与5'端的一个开放阅读框同源，而5 '端的一个开放阅读框与5'端的一个开放阅读框同源。（97~ 936 nt）编码一个279个氨基酸的3a蛋白，另一个位于3 '端，（1225~ 1871 nt）编码218个氨基酸的外壳蛋白，5 '非编码区（NR）、顺反子间区（IR）和3' NR分别为96 nt、288 nt和324 nt，从核苷酸和推测的氨基酸水平上与亚群Ⅱ中的其它株系进行比较，发现Xb的编码区和非编码区的核苷酸序列非常保守，亚群Ⅱ成员的RNA 3s进化是连续的。
The nucleotide sequence of RNA3 of a cucumber mosaic virus strain from banana of subgroup Ⅱ(CMV-Xb) was cloned and analyzed at nucleotide and protein level after RT-PCR amplificaion with two pairs of primers.The results showed that CMV-Xb is composed of 2205 nucleotides(nt) with two open reading frames.The one near 5'termini(97～936nt) encodes a 3a protein with 279 amino acids(aa),the other near 3'termini (1225～1871nt)codes for coat protein with 218aa,The 5'non-coding region(NR),intercistron region(IR) and 3'NR are 96nt,288nt and 324nt, respectively.Comparison at the nucleotide and putative amino acid level with other strains in subgroup Ⅱ showed that the nucleotide sequence of Xb is very conservative in the encoding regions as well as non-coding regions.The evolution of RNA3s of subgroup Ⅱ members of CMV is consecutive.