Increased expression of ribosomal protein S2 in liver tumors, posthepactomized livers, and proliferating hepatocytes in vitro.
Increased expression of ribosomal protein S2 in liver tumors, posthepactomized livers, and proliferating hepatocytes in vitro.
复制标题
DOI：
10.18388/abp.2002_3770
复制
发表时间：
2002
影响因子：
1.7
通讯作者：
P. Kowalczyk;M. Woszczyński;J. Ostrowski
中科院分区：
文献类型：
--
作者：
P. Kowalczyk;M. Woszczyński;J. Ostrowski
核糖体蛋白S2（RPS2）由来自高度保守的哺乳动物重复基因家族LLRep3的基因编码。它参与氨酰转移RNA与核糖体的结合，可能影响mRNA翻译的保真度。这些研究旨在测量细胞增殖增加期间RPS2的表达。使用Western和北方印迹分析，我们发现，RPS 2蛋白及其相应的mRNA的水平较高，在小鼠肝细胞癌，在小鼠肝脏后的三分之一部分肝切除术，并在血清饥饿培养的肝细胞血清治疗。我们的研究表明，RPS2的表达增加与细胞增殖增加相关。然而，这种蛋白质的表达改变是否反映了其参与细胞增殖或代表相关现象仍然是一个需要探索的关键问题。
The ribosomal protein S2 (RPS2) is encoded by a gene from the highly conserved mammalian repetitive gene family LLRep3. It participates in aminoacyl-transfer RNA binding to ribosome, potentially affecting the fidelity of mRNA translation. These studies were designed to measure the expression of RPS2 during increased cell proliferation. Using Western and Northern blot analyses, we found that the levels of RPS2 protein and its corresponding mRNA were higher in mouse hepatocellular carcinoma, in mouse livers after one-third partial hepatectomy, and in serum-starved cultured hepatocytes following serum treatment. Our study shows that the increased expression of RPS2 correlates with increased cell proliferation. However, whether the altered expression of this protein reflects its involvement in cellular proliferation or represents an associated phenomena is still a key question that needs to be explored.