Suppression of cytotoxic effect of mitomycin-C by superoxide dismutase in Fanconi's anemia and dyskeratosis congenita fibroblasts.
Suppression of cytotoxic effect of mitomycin-C by superoxide dismutase in Fanconi's anemia and dyskeratosis congenita fibroblasts.
复制标题
超氧化物歧化酶对范可尼贫血和先天性角化不良成纤维细胞中丝裂霉素 C 的细胞毒性作用的抑制作用。
DOI：
10.1093/carcin/4.7.795
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发表时间：
1983
期刊：
影响因子：
4.7
通讯作者：
Little,JB
中科院分区：
文献类型：
--
作者：
Nagasawa,H;Little,JB
生存曲线测定了几个人二倍体成纤维细胞株与丝裂霉素C（MMC）与或不与超氧化物歧化酶（SOD）伴随孵育处理。这些细胞包括范可尼贫血（FA）、先天性角化不良（DC）（一种与FA相关的疾病）、加德纳综合征（GS）和C组着色性干皮病（XPC）患者的细胞。在两种正常细胞株XPC和GS中，与SOD孵育对MMC诱导的细胞毒性没有影响。虽然GM 2053（FA）和两株DC对MMC的杀伤敏感性中等，但与XPC和GS相似，用0.5 μg/ml MMC与SOD联合处理后，这些细胞株的存活率提高了约2-4倍。MMC敏感细胞株GM 1309（FA）的存活率在加入SOD后显著提高，MMC浓度为0.1 μg/ml时，存活率提高了15倍。在MMC处理的正常细胞中，当存活水平降至接近10− 3时，SOD没有诱导存活的增强。我们认为FA和DC细胞对MMC细胞毒性的超敏反应可能与某些自由基中间体失活的缺陷有关。
Survival curves were determined for several human diploid fibroblast strains treated with mitomycin-C (MMC) with or without concomitant incubation with superoxide dismutase (SOD). These included cells from patients with Fanconi's anemia (FA), dyskeratosis congenita (DC) a disorder related to FA, Gardner's syndrome (GS) and xeroderma pigmentosum grop C (XPC). Incubation with SOD had no effect on MMC-induced cytotoxicity in the two normal cell strains, XPC or GS. Although GM2053 (FA) and two DC strains showed intermediate sensitivity to killing by MMC similar to XPC and GS, the survival of these cell strains was increased approximately 2–4 times after 0.5 μg/ml of MMC by concomitant treatment with SOD. Survival of the most MMC sensitive cell strain GM1309 (FA) was sharply increased by adding SOD; survival was enhanced about 15-fold after treatment with 0.1 μg/ml of MMC. SOD induced no enhancement of survival in MMC treated normal cells at survival levels down to nearly 10−3We propose that the hypersensitivity to MMC cytotoxicity in FA and DC cells may involve among other factors a deficiency in the inactivation of certain free radical intermediates.