Forced integration of Moloney murine leukemia virus DNA with a mutant integration site occurs through recombination with VL30 DNA.
Forced integration of Moloney murine leukemia virus DNA with a mutant integration site occurs through recombination with VL30 DNA.
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莫洛尼鼠白血病病毒 DNA 与突变整合位点的强制整合是通过与 VL30 DNA 重组而发生的。
作者：
Murphy,JE;Goff,SP
逆转录病毒 DNA 整合形成原病毒需要线性活性 DNA 末端存在短反向重复序列。我们之前描述了莫洛尼鼠白血病病毒突变体的构建，该突变体在 U5 末端携带 8 bp 缺失，在建立整合原病毒方面存在缺陷。我们现在使用选择性标记来恢复由携带该突变的逆转录病毒载体从 NIH/3T3 细胞转导后形成的稀有原病毒。对两种这样的原病毒的分析表明，两者都是载体和VL30 DNA之间的重组体，并且产生使得VL30序列可以提供用于整合的完整末端序列。
The integration of retrovital DNA to form the provirus requires the presence of short inverted repeat sequences at the termini of the linear vital DNA. We have previously described the construction of a mutant of Moloney murine leukemia virus, carrying an 8-bp deletion in the U5 terminus, that is defective in establishing the integrated provirus. We have now used a selectable marker to allow recovery of rare proviruses formed after transduction from NIH/3T3 cells by retroviral vectors carrying this mutation. Analysis of two such proviruses showed that both were recombinants between the vector and VL30 DNA and arose such that the VL30 sequences could provide an intact terminal sequence for integration.
正常细胞和恶性肿瘤细胞的生长动力学和生化调节
影响因子：
8.8
作者：
B. Lord
通讯作者：
B. Lord