MOLECULAR BIOLOGY New Dinucleotide and Trinucleotide Microsatellite Marker Resources for Cotton Genome Research
MOLECULAR BIOLOGY New Dinucleotide and Trinucleotide Microsatellite Marker Resources for Cotton Genome Research
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DOI：
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发表时间：
2001
影响因子：
3.9
通讯作者：
O. Umesh;K. Reddy;A. Pepper;S. Saha;J. Jenkins;T. Brooks;Y. Bolek;K. El-Zik
中科院分区：
文献类型：
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作者：
O. Umesh;K. Reddy;A. Pepper;S. Saha;J. Jenkins;T. Brooks;Y. Bolek;K. El-Zik
启动了一项多机构合作计划，以简化微卫星捕获和表征的过程，将微卫星开发为信息分子标记，并向棉花研究界传播标记信息。优化了一种简单高效的生物素捕获方法，并用于捕获超过10，000个片段。在测序的588个片段中，几乎所有片段都含有微卫星重复结构。几种重复类型，包括阿加，GA，CA和ACA。设计引物扩增307个独特的微卫星位点（305个核和两个叶绿体编码）。利用PCR技术从G. hirsutum L. CV. TM-1和Tamcot SP37;巴巴多斯湖CV.皮马3-79和皮马S7在该比较中，74个引物（约占子集的49%）显示出可检测的多态性。与高地G.在多毛类品种中，大约26%的引物表现出种内多态性。多态性广泛分布于各种重复类型和结构（例如，不完全和复合重复）。与其他两个先前衍生的微卫星标记集（BNL，CM）的Redemption是低的，这意味着存在于棉花基因组中的微卫星的总池是足够大的，以满足广泛的基因组作图和标记辅助选择项目的要求。
A collaborative multi-institutional program was initiated to streamline the process of microsatellite capture and characterization, development of microsatellites into informative molecular markers, and dissemination of marker information to the cotton research community. A simple and efficient biotin capture method was optimized and used to capture more than 10,000 fragments. Out of 588 fragments sequenced, nearly all contained a microsatellite repeat structure. Several repeat types were represented, including AGA, GA, CA, and ACA. Primers were designed to amplify 307 unique microsatellite loci (305 nuclear and two chloroplast-encoded). One hundred fifty-two microsatellite loci were amplified from G. hirsutum L. cv. TM-1 and Tamcot SP37, and G. barbadense L. cv. Pima 3-79 and Pima S-7. In this comparison, 74 of the primers (approximately 49% of the subset) showed detectable polymorphism. In a comparison of upland G. hirsutum cultivars, approximately 26% of the primers exhibited intraspecific polymorphism. Polymorphism was widely distributed among the various repeat types and structures (e.g., imperfect and compound repeats). Redundancy with two other previously derived microsatellite marker sets (BNL, CM) was low, implying that the total pool of microsatellites present in the cotton genome is large enough to satisfy the requirements of extensive genome mapping and marker-assisted selection projects.