A screen for genes that function in leg disc regeneration in Drosophila melanogaster
A screen for genes that function in leg disc regeneration in Drosophila melanogaster
复制标题
DOI：
10.1016/j.mod.2007.10.003
复制
发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Schubiger, Gerold
中科院分区：
文献类型：
--
作者：
McClure, Kimberly D.;Schubiger, Gerold
许多不同的动物物种在受伤后再生器官或组织的一部分。然而，负责再生生长的分子在很大程度上仍然未知。本文报道的筛选旨在鉴定在再生中起作用的基因以及与果蝇成虫盘再生密切相关的转决定事件。我们筛选了97个隐性致死P-lacZ增强子陷阱线的两个主要标准的集合：第一，显性修改WG诱导的腿到翅膀transdetermination和第二，激活或抑制的lacZ报告基因在芽基在光盘再生的能力。在97个P-lacZ株系中，我们鉴定了6个基因（Kruppel同源物-1，rpd 3，jing，combgap，Aly和S6激酶），它们同时满足这两个标准。这些基因中的五个抑制，而一个增强，腿到翅膀transdetermination，因此影响光盘再生。其中两个基因，jing和rpd 3，在果蝇发育过程中与多梳组（PcG）和三胸组（trxG）基因的染色质重塑蛋白协同作用，从而将染色质重塑与再生过程联系起来。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
Many diverse animal species regenerate parts of an organ or tissue after injury. However, the molecules responsible for the regenerative growth remain largely unknown. The screen reported here aimed to identify genes that function in regeneration and the transdetermination events closely associated with imaginal disc regeneration using Drosophila melanogaster. We screened a collection of 97 recessive lethal P-lacZ enhancer trap lines for two primary criteria: first, the ability to dominantly modify wg-induced leg-to-wing transdetermination and second, for the activation or repression of the lacZ reporter gene in the blastema during disc regeneration. Of the 97 P-lacZ lines, we identified six genes (Kruppel-homolog-1, rpd3, jing, combgap, Aly and S6 kinase) that met both criteria. Five of these genes suppress, while one enhances, leg-to-wing transdetermination and therefore affects disc regeneration. Two of the genes, jing and rpd3, function in concert with chromatin remodeling proteins of the Polycomb Group (PcG) and trithorax Group (trxG) genes during Drosophila development, thus linking chromatin remodeling with the process of regeneration. (c) 2007 Elsevier Ireland Ltd. All rights reserved.