Influence of solid loading on enzymatic hydrolysis of steam exploded or liquid hot water pretreated olive tree biomass
Influence of solid loading on enzymatic hydrolysis of steam exploded or liquid hot water pretreated olive tree biomass
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DOI：
10.1016/j.procbio.2007.03.012
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发表时间：
2007-06-01
影响因子：
4.4
通讯作者：
Ruiz, Encarnacion
中科院分区：
文献类型：
--
作者：
Cara, Cristbal;Moya, Manuel;Ruiz, Encarnacion
橄榄树修剪生物质，无论是液体热水或蒸汽爆破在选定的条件下预处理，被用作酶水解的底物。将预处理的材料进一步进行碱脱木质素，目的是提高水解产率以及增加预处理原料中的纤维素含量。使用补充有β-葡糖苷酶的商业纤维素酶混合物，使用2至30%（w/v）的固体负载范围，进行预处理的残余物的酶促水解。研究了底物浓度对酶解得率和葡萄糖浓度的影响。有和没有脱木素步骤的比较结果。在高底物浓度（>= 20%）下酶水解是可能的，产生浓缩的葡萄糖溶液（>50 g/L）。然而，预处理残余物的纤维素部分保持不变。(C)2007爱思唯尔有限公司保留所有权利。
Olive tree pruning biomass, pretreated by either liquid hot water or steam explosion under selected conditions, was used as a substrate for enzymatic hydrolysis. The pretreated material was further submitted to alkaline delignification, the objective being to improve hydrolysis yields as well as increasing cellulose content in the pretreated feedstock. The enzymatic hydrolysis of pretreated residues was performed using a commercial cellulase mixture supplemented with beta-glucosidase, using a solid loading range from 2 to 30% (w/v). The influence of substrate concentration on the enzymatic hydrolysis yield and on glucose concentration was studied. Comparative results with and without a delignification step are presented. Enzymatic hydrolysis at high substrate concentration (>= 20%) is possible, yielding a concentrated glucose solution (>50 g/L). Nevertheless, a cellulose fraction of the pretreated residue remains unaltered. (C) 2007 Elsevier Ltd. All rights reserved.