An herbivore elicitor activates the gene for indole emission in maize
An herbivore elicitor activates the gene for indole emission in maize
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DOI：
10.1073/pnas.260499897
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发表时间：
2000-12-19
影响因子：
11.1
通讯作者：
Gierl, A
中科院分区：
文献类型：
--
作者：
Frey, M;Stettner, C;Gierl, A
玉米和其他多种植物物种会释放挥发性化合物来应对食草动物的攻击，这些化合物可以作为化学信号来向食草动物的天敌发出信号。 N-(17-羟基-亚麻酰基)-L-谷氨酰胺是一种激发子成分，已从食草动物害虫甜菜粘虫的反流物中分离出来并进行了化学表征。这种脂肪酸衍生物被称为volicitin，可引发挥发性成分的合成和释放，包括玉米中的萜类化合物和吲哚。在这里，我们报告了一种以前未鉴定的酶，吲哚-3-甘油磷酸裂解酶（IGL），它催化游离吲哚的形成并被volicitin选择性激活。 IGL 的酶特性与 BX1 类似，BX1 是一种玉米酶，可作为二级防御代谢物 DIBOA 和 DIMBOA 的入口点。基因序列分析表明 Igl 和 Bx1 在进化上与色氨酸合酶 α 亚基相关。
Maize and a variety of other plant species release volatile compounds in response to herbivore attack that serve as chemical cues to signal natural enemies of the feeding herbivore. N-(17-hydroxy-linolenoyl)-L-glutamine is an elicitor component that has been isolated and chemically characterized from the regurgitant of the herbivore-pest beet armyworm. This fatty acid derivative, referred to as volicitin, triggers the synthesis and release of Volatile components, including terpenoids and indole in maize. Here we report on a previously unidentified enzyme, indole-3-glycerol phosphate lyase (IGL), that catalyzes the formation of free indole and is selectively activated by volicitin. IGL's enzymatic properties are similar to BX1, a maize enzyme that serves as the entry point to the secondary defense metabolites DIBOA and DIMBOA. Gene-sequence analysis indicates that Igl and Bx1 are evolutionarily related to the tryptophan synthase alpha subunit.