Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet-Triplet Electron Resonance Spectroscopy.

Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet-Triplet Electron Resonance Spectroscopy.
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福斯特共振能量转移与光诱导三重态-三重态电子共振光谱的直接比较。

DOI:
10.1021/jacs.3c04685
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发表时间:
2023-10-25
影响因子:
15
通讯作者:
Bowen, Alice M.
Bowen, Alice M.
中科院分区:
化学1区
文献类型:
--
作者:
Bertran, Arnau;Morbiato, Laura;Sawyer, Jack;Dalla Torre, Chiara;Heyes, Derren J.;Hay, Sam;Timmel, Christiane R.;Di Valentin, Marilena;De Zotti, Marta;Bowen, Alice M.

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为了进行可靠和全面的结构调查,需要利用不同的补充技术。在这里,我们报告说,双三重态自旋/荧光标记使第一个平行距离测量的电子自旋共振(ESR)和福斯特共振能量转移(FRET)上完全相同的分子与正交的发色团,允许直接比较。一种改进的光诱导三重态-三重态电子共振方法与2-色激发,提高了信噪比的数据,并产生一个距离分布,提供更大的洞察力比单一的距离从FRET。
To carry out reliable and comprehensive structural investigations, the exploitation of different complementary techniques is required. Here, we report that dual triplet-spin/fluorescent labels enable the first parallel distance measurements by electron spin resonance (ESR) and Förster resonance energy transfer (FRET) on exactly the same molecules with orthogonal chromophores, allowing for direct comparison. An improved light-induced triplet–triplet electron resonance method with 2-color excitation is used, improving the signal-to-noise ratio of the data and yielding a distance distribution that provides greater insight than the single distance resulting from FRET.
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发表时间: 2021-05-27
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