Modifications of the mouse calvarial technique improve the responsiveness to stimulators of bone resorption
Modifications of the mouse calvarial technique improve the responsiveness to stimulators of bone resorption
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小鼠颅骨技术的改进提高了对骨吸收刺激物的反应性
DOI：
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发表时间：
1987
影响因子：
6.2
通讯作者：
U. Lerner
中科院分区：
文献类型：
--
作者：
U. Lerner
在器官培养系统中，利用新生小鼠的头盖骨，研究了在基础培养液中或在含有前列腺素生物合成抑制剂的培养液中预培养时间对不同骨吸收刺激物的反应的影响。骨吸收通过释放~(45)Ca或从[~3H]-脯氨酸标记的骨中释放~3H来评价。预先孵育的骨在含有或不含有消炎痛、氢化可的松和地塞米松的培养液中培养18-24小时，然后广泛清洗，然后转移到含有不同骨吸收刺激物的培养液中。与解剖后直接暴露于刺激物的骨骼相比，在含有吲哚美辛或皮质类固醇的培养液中预孵育后，骨骼对甲状旁腺激素(PTH)、前列腺素E2(PGE2)、1-α-羟基维生素D3和凝血酶的反应增强。在基础培养液中预先孵育并不能增强随后对PTH的反应。在消炎痛中预先孵育一段时间后，PTH和PGE_2对矿物质动员的刺激作用的剂量-反应曲线可以敏化，而不是新鲜的骨骼。PTH和PGE_2对~(45)Ca释放的作用阈值分别为0.01~0.03和1~3nmol/L。
The effect of a preincubation period, in basic medium or in medium with inhibitors of prostaglandin biosynthesis, on the response to different stimulators of bone resorption has been studied in an organ culture system using calvarial bones from neonatal mice. Bone resorption was assessed either by the release of 45Ca or by the release of 3H from [3H]‐proline labeled bones. Preincubated bones were cultured for 18–24 hr in medium, with and without indomethacin, hydrocortisone, and dexamethasone, and then extensively washed before being transferred to culture medium containing different stimulators of bone resorption. Preincubation in medium containing indomethacin or corticosteroids resulted in an increased response to parathyroid hormone (PTH), prostaglandin E2 (PGE2), 1‐alpha‐hydroxyvitamin‐D3 and thrombin as compared to the response in bones which were exposed to the stimulants directly after dissection. Preincubation in basic medium did not enhance the subsequent response to PTH. By using a preincubation period in indomethacin, the dose‐response curves for the stimulatory effect of PTH and PGE2 on mineral mobilization could be sensitized as compared to the curves obtained with fresh bones. Thus, the concentration of agonists causing 50% stimulation of 45Ca release was decreased by a factor of 10. The threshold for actions of PTH and PGE2 on 45Ca release was 0.01–0.03 and 1–3 nmol/l, respectively.
器官培养中小鼠骨花生四烯酸代谢的环加氧酶产物。
DOI：
10.1016/0005-2760(80)90133-2
发表时间：
1980
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
Voelkel,EF;TashjianJr,AH;Levine,L
通讯作者：
Levine,L